@article{oai:jaxa.repo.nii.ac.jp:00022655,
 author = {立石, 涼介 and 秋山, 伸子 and 宮内, 真紀 and 良永, 理子 and 笹沼, 寛樹 and 工藤, 崇 and 新保, 未来 and 篠原, 正浩 and 小畑, 孝二 and 井上, 純一郎 and 白川, 正輝 and 芝, 大 and 浅原, 弘嗣 and 吉田, 進昭 and 高橋, 智 and 森田, 啓之 and 秋山, 泰身 and Tateishi, Ryosuke and Akiyama, Nobuko and Miyauchi, Maki and Yoshinaga, Riko and Sasanuma, Hiroki and Kudo, Takashi and Shimbo, Miki and Shinohara, Masahiro and Obata, Koji and Inoue, Junichiro and Shirakawa, Masaki and Shiba, Dai and Asahara, Hiroshi and Yoshida, Nobuaki and Takahashi, Satoru and Morita, Hironobu and Akiyama, Taishin},
 issue = {10},
 journal = {PLoS ONE},
 month = {Oct},
 note = {Gravity change affects many immunological systems. We investigated the effects of hypergravity (2G) on murine thymic cells. Exposure of mice to 2G for three days reduced the frequency of CD4(+)CD8(+) thymocytes (DP) and mature medullary thymic epithelial cells (mTECs), accompanied by an increment of keratin-5 and keratin-8 double-positive (K5(+)K8(+)) TECs that reportedly contain TEC progenitors. Whereas the reduction of DP was recovered by a 14-day exposure to 2G, the reduction of mature mTECs and the increment of K5(+)K8(+) TEC persisted. Interestingly, a surgical lesion of the inner ear’s vestibular apparatus inhibited these hypergravity effects. Quantitative PCR analysis revealed that the gene expression of Aire and RANK that are critical for mTEC function and development were upregulated by the 3-day exposure and subsequently down-regulated by the 14-day exposure to 2G. Unexpectedly, this dynamic change in mTEC gene expression was independent of the vestibular apparatus. Overall, data suggest that 2G causes a temporary reduction of DP and a persistent reduction of mature mTECs in a vestibular system-dependent manner, and also dysregulates mTEC gene expression without involving the vestibular system. These data might provide insight on the impact of gravity change on thymic functions during spaceflight and living., 形態: カラー図版あり, Physical characteristics: Original contains color illustrations, 資料番号: PA1610028000},
 title = {Hypergravity Provokes a Temporary Reduction in CD4+CD8+Thymocyte Number and a Persistent Decrease in Medullary Thymic Epithelial Cell Frequency in Mice},
 volume = {10},
 year = {2015}
}