{"created":"2023-06-20T14:54:20.082050+00:00","id":22657,"links":{},"metadata":{"_buckets":{"deposit":"3ca17c3d-6447-4c11-b735-cdb73f34f5be"},"_deposit":{"created_by":1,"id":"22657","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"22657"},"status":"published"},"_oai":{"id":"oai:jaxa.repo.nii.ac.jp:00022657","sets":["1887:1888"]},"author_link":["212373","212393","212381","212380","212377","212382","212379","212366","212387","212370","212375","212385","212389","212391","212383","212390","212384","212374","212378","212386","212371","212376","212367","212388","212372","212368","212369","212392"],"item_7_biblio_info_10":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2015-08-21","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"7","bibliographicVolumeNumber":"1","bibliographic_titles":[{},{"bibliographic_title":"Science Advances","bibliographic_titleLang":"en"}]}]},"item_7_description_17":{"attribute_name":"抄録（英）","attribute_value_mlt":[{"subitem_description":"Hydrolysis of carbohydrates is a major bioreaction in nature, catalyzed by glycoside hydrolases (GHs). We used neutron diffraction and high-resolution x-ray diffraction analyses to investigate the hydrogen bond network in inverting cellulase PcCel45A, which is an endoglucanase belonging to subfamily C of GH family 45, isolated from the basidiomycete Phanerochaete chrysosporium. Examination of the enzyme and enzyme-ligand structures indicates a key role of multiple tautomerizations of asparagine residues and peptide bonds, which are finally connected to the other catalytic residue via typical side-chain hydrogen bonds, in forming the Newton’s cradle - like proton relay pathway of the catalytic cycle. Amide-imidic acid tautomerization of asparagine has not been taken into account in recent molecular dynamics simulations of not only cellulases but also general enzyme catalysis, and it may be necessary to reconsider our interpretation of many enzymatic reactions.","subitem_description_type":"Other"}]},"item_7_description_18":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"形態: カラー図版あり","subitem_description_type":"Other"}]},"item_7_description_19":{"attribute_name":"内容記述（英）","attribute_value_mlt":[{"subitem_description":"Physical characteristics: Original contains color illustrations","subitem_description_type":"Other"}]},"item_7_description_32":{"attribute_name":"資料番号","attribute_value_mlt":[{"subitem_description":"資料番号: PA1610030000","subitem_description_type":"Other"}]},"item_7_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"米国科学振興協会(AAAS)"}]},"item_7_publisher_9":{"attribute_name":"出版者（英）","attribute_value_mlt":[{"subitem_publisher":"American Association for the Advancement of Science"}]},"item_7_relation_25":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"info:doi/10.1126/sciadv.1500263"}],"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"http://dx.doi.org/10.1126/sciadv.1500263","subitem_relation_type_select":"DOI"}}]},"item_7_rights_28":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"2015 © The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC). 10.1126/sciadv.1500263"}]},"item_7_source_id_21":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"2375-2548","subitem_source_identifier_type":"ISSN"}]},"item_7_text_6":{"attribute_name":"著者所属","attribute_value_mlt":[{"subitem_text_value":"東京大学"},{"subitem_text_value":"東京大学"},{"subitem_text_value":"茨城大学フロンティア応用原子科学研究センター"},{"subitem_text_value":"茨城大学フロンティア応用原子科学研究センター"},{"subitem_text_value":"東京大学"},{"subitem_text_value":"茨城大学フロンティア応用原子科学研究センター"},{"subitem_text_value":"琉球大学"},{"subitem_text_value":"宇宙航空研究開発機構きぼう利用センター(JAXA)"},{"subitem_text_value":"株式会社コンフォーカルサイエンス"},{"subitem_text_value":"株式会社丸和栄養食品"},{"subitem_text_value":"兵庫県立大学"},{"subitem_text_value":"茨城大学フロンティア応用原子科学研究センター"},{"subitem_text_value":"東京大学"},{"subitem_text_value":"東京大学"}]},"item_7_text_7":{"attribute_name":"著者所属（英）","attribute_value_mlt":[{"subitem_text_language":"en","subitem_text_value":"The University of Tokyo"},{"subitem_text_language":"en","subitem_text_value":"The University of Tokyo"},{"subitem_text_language":"en","subitem_text_value":"Frontier Research Center for Applied Atomic Sciences, Ibaraki University"},{"subitem_text_language":"en","subitem_text_value":"Frontier Research Center for Applied Atomic Sciences, Ibaraki University"},{"subitem_text_language":"en","subitem_text_value":"The University of Tokyo"},{"subitem_text_language":"en","subitem_text_value":"Frontier Research Center for Applied Atomic Sciences, Ibaraki University"},{"subitem_text_language":"en","subitem_text_value":"University of the Ryukyus"},{"subitem_text_language":"en","subitem_text_value":"Japanese Experiment Module (JEM) Utilization Center, Japan Aerospace Exploration Agency (JAXA)"},{"subitem_text_language":"en","subitem_text_value":"Confocal Science Inc."},{"subitem_text_language":"en","subitem_text_value":"Maruwa Foods and Biosciences Inc."},{"subitem_text_language":"en","subitem_text_value":"University of Hyogo"},{"subitem_text_language":"en","subitem_text_value":"Frontier Research Center for Applied Atomic Sciences, Ibaraki University"},{"subitem_text_language":"en","subitem_text_value":"The University of Tokyo"},{"subitem_text_language":"en","subitem_text_value":"The University of Tokyo"}]},"item_7_version_type_30":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"中村, 彰彦"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"石田, 卓也"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"日下, 勝弘"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"山田, 太郎"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"伏信, 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Taro","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Fushinobu, Shinya","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Tanaka, Ichiro","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kaneko, Satoshi","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Ohta, Kazunori","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Tanaka, Hiroaki","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Inaka, Koji","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Higuchi, Yoshiki","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Niimura, Nobuo","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Samejima, Masahiro","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Igarashi, Kiyohiko","creatorNameLang":"en"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-01-22"}],"displaytype":"detail","filename":"PA1610030000.pdf","filesize":[{"value":"2.9 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"PA1610030000.pdf","url":"https://jaxa.repo.nii.ac.jp/record/22657/files/PA1610030000.pdf"},"version_id":"903cd5a1-4297-436f-9d8e-823f563450a4"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Cellulase","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Carbohydrate Active enZymes","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Proton relay","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Neutron crystallography","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"biomass","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Tautomerization","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Phanerochaete chrysosporium","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"\"Newton's cradle\" proton relay with amide-imidic acid tautomerization in inverting cellulase visualized by neutron crystallography","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"\"Newton's cradle\" proton relay with amide-imidic acid tautomerization in inverting cellulase visualized by neutron crystallography","subitem_title_language":"en"}]},"item_type_id":"7","owner":"1","path":["1888"],"pubdate":{"attribute_name":"公開日","attribute_value":"2016-05-31"},"publish_date":"2016-05-31","publish_status":"0","recid":"22657","relation_version_is_last":true,"title":["\"Newton's cradle\" proton relay with amide-imidic acid tautomerization in inverting cellulase visualized by neutron crystallography"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-06-21T03:17:30.446236+00:00"}