{"created":"2023-06-20T14:54:20.346146+00:00","id":22663,"links":{},"metadata":{"_buckets":{"deposit":"ceac312e-4810-4096-bf47-206ff147f192"},"_deposit":{"created_by":1,"id":"22663","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"22663"},"status":"published"},"_oai":{"id":"oai:jaxa.repo.nii.ac.jp:00022663","sets":["1887:1888"]},"author_link":["212567","212552","212558","212568","212564","212561","212574","212551","212573","212557","212559","212569","212572","212570","212566","212575","212571","212556","212553","212578","212576","212560","212555","212565","212563","212554","212577","212562"],"item_7_biblio_info_10":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2016-05","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"31","bibliographicPageStart":"26","bibliographicVolumeNumber":"195","bibliographic_titles":[{},{"bibliographic_title":"Comparative Biochemistry and Physiology: part A: Molecular & Integrative Physiology","bibliographic_titleLang":"en"}]}]},"item_7_description_17":{"attribute_name":"抄録（英）","attribute_value_mlt":[{"subitem_description":"Using fish scales in which osteoclasts and osteoblasts coexist on the calcified bone matrix, we examined the effects of low-intensity pulsed ultrasound (LIPUS) on both osteoclasts and osteoblasts. At 3 h of incubation after LIPUS treatment, osteoclastic markers such as tartrate-resistant acid phosphatase (TRAP) and cathepsin K mRNA expressions decreased significantly while mRNA expressions of osteoblastic markers, osteocalcin, distal-less homeobox 5, runt-related transcription factor 2a, and runt-related transcription factor 2b, increased significantly. At 6 and 18 h of incubation, however, both osteoclastic and osteoblastic marker mRNA expression did not change at least present conditions. Using GeneChip analysis of zebrafish scales treated with LIPUS, we found that cell death-related genes were upregulated with LIPUS treatment. Real-time PCR analysis indicated that the expression of apoptosis-related genes also increased significantly. To confirm the involvement of apoptosis in osteoclasts with LIPUS, osteoclasts were induced by autotransplanting scales in goldfish. Thereafter, the DNA fragmentation associated with apoptosis was detected in osteoclasts using the TUNEL (TdT-mediated dUTP nick end labeling) method. The multi-nuclei of TRAP-stained osteoclasts in the scales were labeled with TUNEL. TUNEL staining showed that the number of apoptotic osteoclasts in goldfish scales was significantly elevated by treatment with LIPUS at 3 h of incubation. Thus, we are the first to demonstrate that LIPUS directly functions to osteoclasts and to conclude that LIPUS directly causes apoptosis in osteoclasts shortly after exposure.","subitem_description_type":"Other"}]},"item_7_description_32":{"attribute_name":"資料番号","attribute_value_mlt":[{"subitem_description":"資料番号: PA1610038000","subitem_description_type":"Other"}]},"item_7_publisher_9":{"attribute_name":"出版者（英）","attribute_value_mlt":[{"subitem_publisher":"Elsevier"}]},"item_7_relation_25":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"info:doi/10.1016/j.cbpa.2016.01.022"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"http://dx.doi.org/10.1016/j.cbpa.2016.01.022","subitem_relation_type_select":"DOI"}}]},"item_7_source_id_21":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1095-6433","subitem_source_identifier_type":"ISSN"}]},"item_7_source_id_24":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA11525205","subitem_source_identifier_type":"NCID"}]},"item_7_text_6":{"attribute_name":"著者所属","attribute_value_mlt":[{"subitem_text_value":"金沢大学"},{"subitem_text_value":"金沢大学"},{"subitem_text_value":"宇宙航空研究開発機構(JAXA)"},{"subitem_text_value":"富山県立大学"},{"subitem_text_value":"岡山大学"},{"subitem_text_value":"富山大学"},{"subitem_text_value":"富山大学"},{"subitem_text_value":"金沢大学"},{"subitem_text_value":"東京海洋大学"},{"subitem_text_value":"岡山大学"},{"subitem_text_value":"金沢大学"},{"subitem_text_value":"金沢大学"},{"subitem_text_value":"神奈川歯科大学"},{"subitem_text_value":"東京医科歯科大学"}]},"item_7_text_7":{"attribute_name":"著者所属（英）","attribute_value_mlt":[{"subitem_text_language":"en","subitem_text_value":"Kanazawa University"},{"subitem_text_language":"en","subitem_text_value":"Kanazawa University"},{"subitem_text_language":"en","subitem_text_value":"Japan Aerospace Exploration Agency (JAXA)"},{"subitem_text_language":"en","subitem_text_value":"Toyama Prefectural University"},{"subitem_text_language":"en","subitem_text_value":"Okayama University"},{"subitem_text_language":"en","subitem_text_value":"University of Toyama"},{"subitem_text_language":"en","subitem_text_value":"University of Toyama"},{"subitem_text_language":"en","subitem_text_value":"Kanazawa University"},{"subitem_text_language":"en","subitem_text_value":"Tokyo University of Marine Science and Technology"},{"subitem_text_language":"en","subitem_text_value":"Okayama University"},{"subitem_text_language":"en","subitem_text_value":"Kanazawa University"},{"subitem_text_language":"en","subitem_text_value":"Kanazawa University"},{"subitem_text_language":"en","subitem_text_value":"Kanagawa Dental University Graduate School of Dentistry"},{"subitem_text_language":"en","subitem_text_value":"Tokyo Medical and Dental University"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"鈴木, 信雄"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"半本, 泰三"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"矢野, 幸子"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"古澤, 之裕"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"池亀, 美華"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"田渕, 圭章"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"近藤, 隆"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"北村, 敬一郎"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"遠藤, 雅人"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"山本, 敏男"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"関口, 俊男"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"浦田, 慎"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Mikuni高垣, 裕子"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"服部, 淳彦"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Suzuki, Nobuo","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Hanmoto, Taizo","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Yano, Sachiko","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Furusawa, Yukihiro","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Ikegame, Mika","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Tabuchi, Yoshiaki","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kondo, Takashi","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kitamura, Keiichiro","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Endo, Masato","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Yamamoto, Toshio","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Sekiguchi, Toshio","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Urata, Makoto","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Mikuni-Takagaki, Yuko","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Hattori, Atsuhiko","creatorNameLang":"en"}],"nameIdentifiers":[{}]}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"LIPUS","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Osteoclasts","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Osteoblasts","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Fish scales","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Apoptosis","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"GeneChip analysis","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Low-intensity pulsed ultrasound induces apoptosis in osteoclasts: Fish scales are a suitable model for the analysis of bone metabolism by ultrasound","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Low-intensity pulsed ultrasound induces apoptosis in osteoclasts: Fish scales are a suitable model for the analysis of bone metabolism by ultrasound","subitem_title_language":"en"}]},"item_type_id":"7","owner":"1","path":["1888"],"pubdate":{"attribute_name":"公開日","attribute_value":"2016-06-13"},"publish_date":"2016-06-13","publish_status":"0","recid":"22663","relation_version_is_last":true,"title":["Low-intensity pulsed ultrasound induces apoptosis in osteoclasts: Fish scales are a suitable model for the analysis of bone metabolism by ultrasound"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-06-21T03:17:25.582087+00:00"}