@article{oai:jaxa.repo.nii.ac.jp:00034713,
 author = {渡辺, 格 and 川出, 由己 and 中村, 正好 and WATANABE, Itaru and KAWADE, Yoshimi and NAKAMURA, Masayoshi},
 issue = {3},
 journal = {東京大學理工學研究所報告},
 month = {Jul},
 note = {Procedures for the purification of tobacco moasic virus (TMV) from tobacco leaves by several cycles of alternate high and low speed centrifugations using an air-driven ultracentrifuge first constructed in Japan recently are described in detail. Colored impurities were easily removed when White Burley tobacco plants were used as the starting material. The solvents used for dissolving pellets of TMV were mainly 0.1M phosphate buffer at pH7.0, followed by 0.01M buffer. The properties of the purified products were examined by various means including biological assay (preliminary) , chemical analyses, electrophoresis, diffusion, sedimentation velocity, viscosity and electron micrography. It was shown that chemical purity was satisfactory, but the homogeneity with respect to the length of the TMV particle was not always good, as judged by electron micrographs and sedimentation patterns. Especially, rod particles shorter than "monomer" (300mμ in length) were always observed in electron micrographs, sometimes existing in large proportions, and the possible causes of their occurrence were discussed. It is presumed that some of them are produced during preparation of the specimen for the electron microscopic observation. Some have probably existed in the original juice. Although a preparation showed a very sharp peak in sedimentation pattern, another preparation, which was purified carefully, revealed two components in sedimentation measurements performed immediately after the purification. Thus, further investigations seem necessary to establish a reliable method to obtain highly homogeneous preparations., 資料番号: SA2410210000},
 pages = {99--113},
 title = {超遠心機によるタバコ・モザイク・バイラスの精製の研究},
 volume = {8},
 year = {1954}
}