@article{oai:jaxa.repo.nii.ac.jp:00034714,
 author = {川出, 由己 and KAWADE, Yoshimi},
 issue = {3},
 journal = {東京大學理工學研究所報告},
 month = {Jul},
 note = {タバコ・モザイク・バイラス(TMV)は細長い棒状の粒子で,條件によつて容易に会合や分裂を起すことが知られているが,その溶液の粘度はTMV粒子の軸比の変化に対して敏感であるので,粘度測定による粒子の分散状態の研究はしばしば才了われている.我々はさきに報告したようにTMVを超遠心機で精製することに成功し・精製した試料について沈降速度,拡散その他の測定を行って来たが,その中で一つの試料の粘度について興味ある現象が見出されたのでここに報告する., Viscosity of solutions of tobacco mosaic virus (TMV) was measured by Ostwald-type viscometers with maximum velocity gradients of flow of about 100 sec^<-1> which make the effect of orientation of rod-like particles of TMV negligibly small. The viscosity of TMV solutions in 0.01M phosphate buffer at pH7.0 decreased slowly when the solutions examined were stored at a temperature near 0℃ during several months after purification, and approached the probable stationary values which correspond to intrinsic viscosity of about 50(cc/cc). At concentrations higher than 1%, the flow time increased steadily during measurement at 18° or 30℃, the rate of increase diminishing as the over-all relative viscosity lowered during storage in the cold. These phenomena are temporarily interpreted in terms of disaggregation while cold storage and aggregation at higher concentration and high temperature. In another series of experiments performed at about five months after the purification, phosphate was added to the stock solution of TMV (in 0.01M phosphate buffer at pH7.0) so as to make the phosphate concentration 0.1M. The solution showed very high viscosity, indicating marked end-to-end aggregation of virus particles. The relative viscosity tended to increase when these solutions were stored at room temperature, and to decrease when stored in the cold. Thus, the importance of specifying the history of the solution (particularly concerning the temperature) in determining the size and shape of particles of TMV is emphasized. In the present experiment the solvent used for preservation of purified TMV (0.01M phosphate buffer at pH7.0) may not be the best., 資料番号: SA2410211000},
 pages = {115--120},
 title = {タバコ・モザイク・バイラス溶液の粘度},
 volume = {8},
 year = {1954}
}