{"created":"2023-06-20T15:10:07.385346+00:00","id":39714,"links":{},"metadata":{"_buckets":{"deposit":"489c3e26-0e17-4d32-a825-cd8434e3b673"},"_deposit":{"created_by":1,"id":"39714","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"39714"},"status":"published"},"_oai":{"id":"oai:jaxa.repo.nii.ac.jp:00039714","sets":["1887:1893","1896:1898:1933:1934"]},"author_link":["500024","500021","500017","500019","500023","500008","500020","500015","500010","500013","500018","500022","500009","500014","500016","500011","500007","500012","500005","500006"],"item_3_alternative_title_2":{"attribute_name":"その他のタイトル（英）","attribute_value_mlt":[{"subitem_alternative_title":"Effects of microgravity on cell growth and differentiated function of cultured osteoblast (Osteogene)"}]},"item_3_biblio_info_10":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1996-03-15","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"151","bibliographicPageStart":"141","bibliographic_titles":[{"bibliographic_title":"宇宙開発事業団技術報告"},{"bibliographic_title":"NASDA Technical Memorandum","bibliographic_titleLang":"en"}]}]},"item_3_description_16":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"宇宙飛行士の尿中カルシウム量の増大、骨密度の減少、骨無機質の脱落が報告されている。2-3週間宇宙旅行したラットでの骨形成の阻害の報告もある。こうしたことは微小重力が骨代謝に影響を及ぼすことを示唆しているが、そのメカニズムは解明されていない。本研究の目的は、微小重力が骨由来培養細胞に及ぼす影響のメカニズムを明らかにすることである。実験は、遺伝子発現と細胞産生能、サイトカイン、増殖因子、骨基質タンパク質に注目しておこなった。遺伝子発現はリバーストランスクリプターゼ-ポリメラーゼ連鎖反応(RT-PCR)により定量分析し、骨由来細胞の分化機能は、酵素免疫測定法や同位元素標識免疫定量法を使用して細胞産生物質を定量的に調査した。培地中に分泌される細胞産生物質は宇宙フライト中に採取し、地球に帰還後、定量分析をおこなった。微小重力下では、プロスタグランジンE2産生は2-10倍に増加したが、オステオカルシン産生は60-90%抑制された。プロスタグランジンE2は破骨細胞の誘導を活性化し、オステオカルシンは骨基質タンパク質であるヒドロキシアパタイト結晶形成に何らかの役割を果たしていることが報告されている。細胞産生能の変化は、プロスタグランジンE2およびオステオカルシン合成に対応するmRNAレベルでの変化と一致していた。微小重力が、破骨細胞の分化した機能に転写レベルで作用していること、および細胞産生物質の変化が、微小重力下で次に骨無機質脱落を誘導するであろうということを、これらの結果は示唆している。","subitem_description_type":"Abstract"}]},"item_3_description_17":{"attribute_name":"抄録（英）","attribute_value_mlt":[{"subitem_description":"The increase of urinary calcium and decrease of bone density, bone demineralization have been reported in astronauts. Inhibition of bone formation was reported in rats that were flown for two to three weeks. It has been suggested that microgravity affects bone metabolism, however, the mechanism remains unknown. The purpose of this study is to clarify the mechanism of microgravity effects on cultured osteoblasts. The investigation is focused on the gene expression and cell productivity, cytokines, growth factors, and bone matrix proteins. Gene expression was analyzed by quantitative Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR), whereas differentiated function of osteoblasts was investigated by quantitating cellular products using enzyme immunoassay or radioimmunoassay. The cellular products which were secreted into the culture medium were harvested during space flight and analyzed quantitatively after return to the earth. In microgravity, prostaglandin E2 production was increased by two- to ten-fold, whereas osteocalcin productivity was inhibited by 60 to 90 percent. Prostaglandin E2 is reported to activate on osteoclast induction, whereas osteocalcin is reported to play some role in hydroxyapatite crystal formation as the bone matrix protein. The changes of cellular productivity were consistent with changes in mRNA (m Ribonucleic Acid) levels for prostaglandin E2 and osteocalcin synthesis. These results suggest that microgravity affects differentiated function of osteoblasts at the transcriptional level and that the changes of the cellular products will then induce bone demineralization in microgravity.","subitem_description_type":"Other"}]},"item_3_description_32":{"attribute_name":"資料番号","attribute_value_mlt":[{"subitem_description":"資料番号: AA0000271005","subitem_description_type":"Other"}]},"item_3_description_33":{"attribute_name":"レポート番号","attribute_value_mlt":[{"subitem_description":"レポート番号: NASDA-TMR-960004","subitem_description_type":"Other"}]},"item_3_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"宇宙開発事業団"}]},"item_3_publisher_9":{"attribute_name":"出版者（英）","attribute_value_mlt":[{"subitem_publisher":"National Space Development Agency of Japan (NASDA)"}]},"item_3_source_id_21":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1345-7888","subitem_source_identifier_type":"ISSN"}]},"item_3_source_id_24":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AN00364784","subitem_source_identifier_type":"NCID"}]},"item_3_text_6":{"attribute_name":"著者所属","attribute_value_mlt":[{"subitem_text_value":"東京医科歯科大学 歯学部"},{"subitem_text_value":"東京医科歯科大学 歯学部"},{"subitem_text_value":"大阪大学 医学部"},{"subitem_text_value":"東レリサーチセンター 生物科学研究部"},{"subitem_text_value":"東レリサーチセンター 生物科学研究部"},{"subitem_text_value":"北海道大学 歯学部"},{"subitem_text_value":"北海道大学 歯学部"},{"subitem_text_value":"宇宙開発事業団 筑波宇宙センター"},{"subitem_text_value":"宇宙開発事業団 筑波宇宙センター"},{"subitem_text_value":"NASA Johnson Space Center"}]},"item_3_text_7":{"attribute_name":"著者所属（英）","attribute_value_mlt":[{"subitem_text_language":"en","subitem_text_value":"Tokyo Medical and Dental University Faculty of Dentistry"},{"subitem_text_language":"en","subitem_text_value":"Tokyo Medical and Dental University Faculty of Dentistry"},{"subitem_text_language":"en","subitem_text_value":"Osaka University Medical School"},{"subitem_text_language":"en","subitem_text_value":"Toray Research Center Inc Department of Biological Science"},{"subitem_text_language":"en","subitem_text_value":"Toray Research Center Inc Department of Biological Science"},{"subitem_text_language":"en","subitem_text_value":"Hokkaido University School of Dentistry"},{"subitem_text_language":"en","subitem_text_value":"Hokkaido University School of Dentistry"},{"subitem_text_language":"en","subitem_text_value":"National Space Development Agency of Japan Tsukuba Space Center"},{"subitem_text_language":"en","subitem_text_value":"National Space Development Agency of Japan Tsukuba Space Center"},{"subitem_text_language":"en","subitem_text_value":"NASA Johnson Space Center"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"粂井, 康宏"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"下川, 仁弥太"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"原, 英二"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"秋山, 英雄"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"平野, 昌彦"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"藤沢, 隆一"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"久保木, 芳徳"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"向井, 千秋"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"長岡, 俊治"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Whitson, P.A."}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kumei, Yasuhiro","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Shimokawa, Hitoyata","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Hara, Eiji","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Akiyama, Hideo","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Hirano, Masahiko","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Fujisawa, Ryuichi","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kuboki, Yoshinori","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Mukai, Chiaki","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Nagaoka, Shunji","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Whitson, Peggy A.","creatorNameLang":"en"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-02-10"}],"displaytype":"detail","filename":"00271005.pdf","filesize":[{"value":"937.5 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"00271005.pdf","url":"https://jaxa.repo.nii.ac.jp/record/39714/files/00271005.pdf"},"version_id":"6bd62ec8-1780-4252-be91-7111ab5cfe85"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"骨由来培養細胞","subitem_subject_scheme":"Other"},{"subitem_subject":"微小重力","subitem_subject_scheme":"Other"},{"subitem_subject":"無機質脱落","subitem_subject_scheme":"Other"},{"subitem_subject":"遺伝子発現","subitem_subject_scheme":"Other"},{"subitem_subject":"プロスタグランジン","subitem_subject_scheme":"Other"},{"subitem_subject":"オステオカルシン","subitem_subject_scheme":"Other"},{"subitem_subject":"破骨細胞","subitem_subject_scheme":"Other"},{"subitem_subject":"リバーストランスクリプターゼポリメラーゼ連鎖反応","subitem_subject_scheme":"Other"},{"subitem_subject":"RT-PCR","subitem_subject_scheme":"Other"},{"subitem_subject":"宇宙実験","subitem_subject_scheme":"Other"},{"subitem_subject":"細胞産生能","subitem_subject_scheme":"Other"},{"subitem_subject":"サイトカイン","subitem_subject_scheme":"Other"},{"subitem_subject":"増殖因子","subitem_subject_scheme":"Other"},{"subitem_subject":"骨基質タンパク質","subitem_subject_scheme":"Other"},{"subitem_subject":"ラット","subitem_subject_scheme":"Other"},{"subitem_subject":"cultured osteoblast","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"microgravity","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"demineralization","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"gene expression","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"prostaglandin","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"osteocalcin","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"osteoclast","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"reverse transcriptase polimerase chain reaction","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"RT PCR","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"space experiment","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"cell productivity","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"cytokine","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"growth factor","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"bone matrix protein","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"rat","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"technical report","resourceuri":"http://purl.org/coar/resource_type/c_18gh"}]},"item_title":"骨由来培養細胞の増殖・分化機能発現に及ぼす微小重力の影響(OSTEOGENE)","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"骨由来培養細胞の増殖・分化機能発現に及ぼす微小重力の影響(OSTEOGENE)"}]},"item_type_id":"3","owner":"1","path":["1893","1934"],"pubdate":{"attribute_name":"公開日","attribute_value":"2015-03-26"},"publish_date":"2015-03-26","publish_status":"0","recid":"39714","relation_version_is_last":true,"title":["骨由来培養細胞の増殖・分化機能発現に及ぼす微小重力の影響(OSTEOGENE)"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-06-20T20:29:52.104089+00:00"}