{"created":"2023-06-20T15:10:07.533531+00:00","id":39717,"links":{},"metadata":{"_buckets":{"deposit":"799af05d-d552-407f-9f85-106784da21bc"},"_deposit":{"created_by":1,"id":"39717","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"39717"},"status":"published"},"_oai":{"id":"oai:jaxa.repo.nii.ac.jp:00039717","sets":["1887:1893","1896:1898:1933:1934"]},"author_link":["500035","500036","500038","500037","500041","500034","500039","500040"],"item_3_alternative_title_2":{"attribute_name":"その他のタイトル（英）","attribute_value_mlt":[{"subitem_alternative_title":"Electrophoretic separation of a nematode c. elegans chromosomal DNA (Chromosome)"}]},"item_3_biblio_info_10":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1996-03-15","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"192","bibliographicPageStart":"179","bibliographic_titles":[{"bibliographic_title":"宇宙開発事業団技術報告"},{"bibliographic_title":"NASDA Technical Memorandum","bibliographic_titleLang":"en"}]}]},"item_3_description_16":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"線虫C.elegans DNAの無担体電気泳動(FFE)を、スペースシャトルフライトSTS65/コロンビアの宇宙実験室の電気泳動装置(FFEU)でおこなった。電気泳動実験は微小重力下で搭乗科学技術者がおこなった。実験中、FFEUのハウスキーピングデータとUV検出器による電気泳動移動画像をダウンリンクし、アラバマのマーシャル宇宙飛行センターにあるペイロード運用管制センターで、リアルタイムダウンリンクシステムを使ってモニターした。3次元電気泳動図として観察される移動マーカーの分離画像は、地上で得られたものと比較すると大変安定しており、このことは微小重力下では熱対流による電気泳動移動の乱れが生じないことを示している。さらに、3次元エレクトロフェログラムは本試料の分離行動の評価を可能にし、必要な試料分画を搭乗科学技術者に集めてもらうよう要請することを可能にした。分画された試料の成分DNAのフライト後分析は、DNAが効果的に2つのピークに分けられていることを示した。そのうちのの1つは、PCR法により増幅後、連鎖3および10にそれぞれ対応するsod-4ジーンプローブ対unc-6プローブの比率が7対1のDNA成分を含んでいる。もう片方もその比率は同じである。この分離技術は、DNAなどの生体高分子を分離するのに適しており、微小重力環境下でのFFEの分離能は極めて高いことを、これらの結果は示唆している。","subitem_description_type":"Abstract"}]},"item_3_description_17":{"attribute_name":"抄録（英）","attribute_value_mlt":[{"subitem_description":"Free flow electrophoresis of a nematode C. elegans DNA (Deoxyribonucleic Acid) was carried out using a Free Flow Electrophoresis Unit (FFEU) on the spacelab of a space shuttle flight STS65/Colombia. The electrophoresis experiment were performed by payload specialists under microgravity. During the experiment, the house keeping data of the FFEU and the electrophoretic migration profiles by a UV (Ultraviolet) detector were down-linked and monitored at POCC (Payload Operations Control Center) of MSFC (Marshall Space Flight Center, Alabama) according to the real-time down-link system. Separation profiles of migration markers observed as the three Dimensional Electropherogram (3DEP) were very stable in comparison with that obtained on the ground, indicating that disturbance of electrophoretic migration caused by thermal convection did not occur under microgravity. Furthermore, the 3DEP made it possible to assess the separation behavior of the samples and then to request the necessary sample fractions to be collected by payload specialists. Post-flight analyses of the component DNA (Deoxyribonucleic Acid) of the fractionated samples revealed that the DNAs were separated effectively into two peaks: one of them contained seven folds of a DNA component estimated by a sod-4 gene probe against an unc-6 probe on linkage group three and ten, respectively, after the amplification by the PCR method. The others were the same in that ratio. These results suggested that this separation technique was suitable for separating biological macromolecules such as DNAs, and the separation efficiency of the FFE (Free Flow Electrophoresis) under microgravity environment was very high.","subitem_description_type":"Other"}]},"item_3_description_32":{"attribute_name":"資料番号","attribute_value_mlt":[{"subitem_description":"資料番号: AA0000271008","subitem_description_type":"Other"}]},"item_3_description_33":{"attribute_name":"レポート番号","attribute_value_mlt":[{"subitem_description":"レポート番号: NASDA-TMR-960004","subitem_description_type":"Other"}]},"item_3_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"宇宙開発事業団"}]},"item_3_publisher_9":{"attribute_name":"出版者（英）","attribute_value_mlt":[{"subitem_publisher":"National Space Development Agency of Japan (NASDA)"}]},"item_3_source_id_21":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1345-7888","subitem_source_identifier_type":"ISSN"}]},"item_3_source_id_24":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AN00364784","subitem_source_identifier_type":"NCID"}]},"item_3_text_6":{"attribute_name":"著者所属","attribute_value_mlt":[{"subitem_text_value":"城西大学 理学部"},{"subitem_text_value":"東海大学 医学部"},{"subitem_text_value":"広島大学 工学部"},{"subitem_text_value":"宇宙開発事業団"}]},"item_3_text_7":{"attribute_name":"著者所属（英）","attribute_value_mlt":[{"subitem_text_language":"en","subitem_text_value":"Josai University Faculty of Science"},{"subitem_text_language":"en","subitem_text_value":"Tokai University School of Medicine"},{"subitem_text_language":"en","subitem_text_value":"Hiroshima University Faculty of Engineering"},{"subitem_text_language":"en","subitem_text_value":"National Space Development Agency of Japan"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"小林, 英三郎"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"石井, 直明"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"広川, 健"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"長岡, 俊治"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kobayashi, Hidesaburo","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Ishii, Naoaki","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Hirokawa, Takeshi","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Nagaoka, Shunji","creatorNameLang":"en"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-02-10"}],"displaytype":"detail","filename":"00271008.pdf","filesize":[{"value":"1.2 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"00271008.pdf","url":"https://jaxa.repo.nii.ac.jp/record/39717/files/00271008.pdf"},"version_id":"4fd25586-b1ac-4258-aabb-6d8abf8d02bc"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"線虫","subitem_subject_scheme":"Other"},{"subitem_subject":"FFEU","subitem_subject_scheme":"Other"},{"subitem_subject":"電気泳動装置","subitem_subject_scheme":"Other"},{"subitem_subject":"搭乗科学技術者","subitem_subject_scheme":"Other"},{"subitem_subject":"分離","subitem_subject_scheme":"Other"},{"subitem_subject":"生体高分子","subitem_subject_scheme":"Other"},{"subitem_subject":"DNA","subitem_subject_scheme":"Other"},{"subitem_subject":"FFE","subitem_subject_scheme":"Other"},{"subitem_subject":"無担体電気泳動","subitem_subject_scheme":"Other"},{"subitem_subject":"ダウンリンク","subitem_subject_scheme":"Other"},{"subitem_subject":"3次元エレクトロフェログラム","subitem_subject_scheme":"Other"},{"subitem_subject":"3DEP","subitem_subject_scheme":"Other"},{"subitem_subject":"宇宙実験","subitem_subject_scheme":"Other"},{"subitem_subject":"微小重力","subitem_subject_scheme":"Other"},{"subitem_subject":"nematode","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"FFEU","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"free flow electrophoresis unit","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"payload specialist","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"separation","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"biological macromolecule","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"DNA","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"FFE","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"free flow electrophoresis","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"down link","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"3 dimensional electro pherogram","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"3DEP","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"space experiment","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"microgravity","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"technical report","resourceuri":"http://purl.org/coar/resource_type/c_18gh"}]},"item_title":"電気泳動による線虫C.elegansの染色体DNAの分離(CHROMOSOME)","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"電気泳動による線虫C.elegansの染色体DNAの分離(CHROMOSOME)"}]},"item_type_id":"3","owner":"1","path":["1893","1934"],"pubdate":{"attribute_name":"公開日","attribute_value":"2015-03-26"},"publish_date":"2015-03-26","publish_status":"0","recid":"39717","relation_version_is_last":true,"title":["電気泳動による線虫C.elegansの染色体DNAの分離(CHROMOSOME)"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-06-20T20:29:47.201079+00:00"}