{"created":"2023-06-20T15:10:07.631836+00:00","id":39719,"links":{},"metadata":{"_buckets":{"deposit":"8cfc2db9-4b83-40c5-8afb-596c5168f49c"},"_deposit":{"created_by":1,"id":"39719","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"39719"},"status":"published"},"_oai":{"id":"oai:jaxa.repo.nii.ac.jp:00039719","sets":["1887:1893","1896:1898:1933:1934"]},"author_link":["500058","500064","500060","500056","500063","500061","500055","500062","500059","500057","500065","500054"],"item_3_alternative_title_1":{"attribute_name":"その他のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"電気泳動によるラット脳下垂体からの細胞と粒子の分離(PITUITARY)"}]},"item_3_biblio_info_10":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1996-03-15","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"220","bibliographicPageStart":"205","bibliographic_titles":[{"bibliographic_title":"宇宙開発事業団技術報告"},{"bibliographic_title":"NASDA Technical Memorandum","bibliographic_titleLang":"en"}]}]},"item_3_description_16":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"本稿ではSTS-65でおこなわれた連続流電気泳動(CFE)実験の結果を述べる。微小重力下で、ラット培養前脳下垂体細胞から調製された細胞溶解産物に含まれる成長ホルモン(GH)粒子は、同一速度で移動する地上対照細胞溶解産物とは違った電気泳動移動度を示す可能性があると考え、実験をおこなった。微小重力下では、地上でおこなうよりもCFE処理はうまくおこなえることを、結果は示唆している。つまり、単位時間当りにかなり多くの試料が処理でき(6倍)、そのうえ多様なGH分子を分離できる可能性である。脳下垂体細胞1個のCFE実験をしたいと考えていたが、ハードウエアの故障から実際の電気泳動は、シャトル着地後間もなく地上でおこなうことを余儀なくされた。これらの実験から得られたデータは、宇宙飛行を経験した細胞は、地上の対照細胞に比べ、電気泳動上より多く移動することを示しており、それにより培養細胞を微小重力にさらすと細胞の正味の表面電荷を変化させるー特に細胞が培養されている場合にーという考えに証拠を提供している。この脳下垂体実験から得た結果は、微小重力環境において、細胞培養とCFE技術を組み合わせて使用することの有利さを、実証している。","subitem_description_type":"Abstract"}]},"item_3_description_17":{"attribute_name":"抄録（英）","attribute_value_mlt":[{"subitem_description":"This report describes the results of a Continuous Flow Electrophoresis (CFE) experiment done on STS-65 in which the idea that intracellular Growth Hormone (GH) particles contained in a cell lysate prepared from cultured rat anterior pituitary cells in microgravity might have different electrophoretic mobilities from those in a synchronous ground control cell lysate was tested. Collectively, the results suggested that CFE processing in microgravity was better than on earth; more sample could be processed/time (6x) and more variant forms of GH molecules could be resolved as well. The doing of a pituitary cell CFE experiment had been also hoped, but failure of the hardware required that the actual cell electrophoresis trials be done on earth shortly after Shuttle landing. Data from these experiments showed that spaceflown cells were more electrophoretically mobile than ground control cells, thereby offering evidence for the idea that exposure of cultured cells to microgravity can change their net surface charge, especially when the cells are fed. Collectively, the results from this pituitary cell experiment document the advantage of using coupled cell culture and CFE techniques in the microgravity environment.","subitem_description_type":"Other"}]},"item_3_description_32":{"attribute_name":"資料番号","attribute_value_mlt":[{"subitem_description":"資料番号: AA0000271010","subitem_description_type":"Other"}]},"item_3_description_33":{"attribute_name":"レポート番号","attribute_value_mlt":[{"subitem_description":"レポート番号: NASDA-TMR-960004","subitem_description_type":"Other"}]},"item_3_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"宇宙開発事業団"}]},"item_3_publisher_9":{"attribute_name":"出版者（英）","attribute_value_mlt":[{"subitem_publisher":"National Space Development Agency of Japan (NASDA)"}]},"item_3_source_id_21":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1345-7888","subitem_source_identifier_type":"ISSN"}]},"item_3_source_id_24":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AN00364784","subitem_source_identifier_type":"NCID"}]},"item_3_text_6":{"attribute_name":"著者所属","attribute_value_mlt":[{"subitem_text_value":"Pennsylvania State University Dept. Biochemistry & Molecular Biology"},{"subitem_text_value":"Pennsylvania State University Dept. Biochemistry & Molecular Biology"},{"subitem_text_value":"Pennsylvania State University Dept. Biochemistry & Molecular Biology"},{"subitem_text_value":"Pennsylvania State University Dept. Biochemistry & Molecular Biology"},{"subitem_text_value":"Emerald Diagnostics"},{"subitem_text_value":"宇宙開発事業団"}]},"item_3_text_7":{"attribute_name":"著者所属（英）","attribute_value_mlt":[{"subitem_text_language":"en","subitem_text_value":"Pennsylvania State University Department of Biochemistry and Molecular Biology"},{"subitem_text_language":"en","subitem_text_value":"Pennsylvania State University Department of Biochemistry and Molecular Biology"},{"subitem_text_language":"en","subitem_text_value":"Pennsylvania State University Department of Biochemistry and Molecular Biology"},{"subitem_text_language":"en","subitem_text_value":"Pennsylvania State University Department of Biochemistry and Molecular Biology"},{"subitem_text_language":"en","subitem_text_value":"Emerald Diagnostics"},{"subitem_text_language":"en","subitem_text_value":"National Space Development Agency of Japan"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Hymer, W.C."}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Salada, T."}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Cenci, R."}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Krishnan, K."}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Seaman, G."}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"長岡, 俊治"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Hymer, W. C.","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Salada, T.","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Cenci, R.","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Krishnan, K.","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Seaman, G.","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Nagaoka, Shunji","creatorNameLang":"en"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-02-10"}],"displaytype":"detail","filename":"00271010.pdf","filesize":[{"value":"1.6 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"00271010.pdf","url":"https://jaxa.repo.nii.ac.jp/record/39719/files/00271010.pdf"},"version_id":"a473c484-a2e0-4198-8196-ce19dcbfe3ee"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"ラット","subitem_subject_scheme":"Other"},{"subitem_subject":"脳下垂体","subitem_subject_scheme":"Other"},{"subitem_subject":"GH","subitem_subject_scheme":"Other"},{"subitem_subject":"成長ホルモン","subitem_subject_scheme":"Other"},{"subitem_subject":"CFE","subitem_subject_scheme":"Other"},{"subitem_subject":"連続流電気泳動","subitem_subject_scheme":"Other"},{"subitem_subject":"微小重力","subitem_subject_scheme":"Other"},{"subitem_subject":"培養細胞","subitem_subject_scheme":"Other"},{"subitem_subject":"宇宙実験","subitem_subject_scheme":"Other"},{"subitem_subject":"粒子","subitem_subject_scheme":"Other"},{"subitem_subject":"rat","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"pituitary","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"GH","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"growth hormone","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"CFE","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"continuous flow electrophoresis","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"microgravity","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"cultured cell","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"space experiment","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"particle","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"technical report","resourceuri":"http://purl.org/coar/resource_type/c_18gh"}]},"item_title":"Electrophoretic separation of cells and particles from rat pituitary (Pituitary)","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Electrophoretic separation of cells and particles from rat pituitary (Pituitary)","subitem_title_language":"en"}]},"item_type_id":"3","owner":"1","path":["1893","1934"],"pubdate":{"attribute_name":"公開日","attribute_value":"2015-03-26"},"publish_date":"2015-03-26","publish_status":"0","recid":"39719","relation_version_is_last":true,"title":["Electrophoretic separation of cells and particles from rat pituitary (Pituitary)"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-06-20T20:29:44.483847+00:00"}