{"created":"2023-06-20T15:10:07.832710+00:00","id":39723,"links":{},"metadata":{"_buckets":{"deposit":"84eb93a6-c0f7-452d-aefc-cc784a4dfb96"},"_deposit":{"created_by":1,"id":"39723","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"39723"},"status":"published"},"_oai":{"id":"oai:jaxa.repo.nii.ac.jp:00039723","sets":["1887:1893","1896:1898:1933:1934"]},"author_link":["500094","500096","500095","500105","500100","500098","500093","500092","500104","500099","500097","500103","500102","500101"],"item_3_alternative_title_2":{"attribute_name":"その他のタイトル（英）","attribute_value_mlt":[{"subitem_alternative_title":"Dosimetry measurement of heavy particle beam in a space shuttle and investigation of its effect on living organisms (Radiation): Effect on Escherichia coli and plasmid DNA"}]},"item_3_biblio_info_10":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1996-03-15","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"257","bibliographicPageStart":"251","bibliographic_titles":[{"bibliographic_title":"宇宙開発事業団技術報告"},{"bibliographic_title":"NASDA Technical Memorandum","bibliographic_titleLang":"en"}]}]},"item_3_description_16":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"第2次国際微小重力実験室(IML-2)プロジェクト中に実施された宇宙実験に参加した。本実験は、高線エネルギー付与(LET)宇宙放射線の生物に対する影響を調査するためのもので、リアルタイム放射線モニター装置(RRMD)と放射線線量計を積載し、フライト後の分析もおこなった。生物試料の調製法の1つとして、紫外線照射した大腸菌を使用する予備実験を、地上でおこなった。乾燥させたDNA修復能欠損突然変異体の大腸菌とシャトルベクタープラズミドpZ189DNAの生物試料を調製後、線量計内と放射線モニター装置内に収めたが、後者はHarzlasプラスチック放射線検出器にはさまれたBiospecimen Boxである。これをスペースシャトル「コロンビア」に積載し、また同一の箱をNASAケネディ宇宙センターに地上対照として設置した。「コロンビア(フライト番号STS-65)」は1994年7月9日に合衆国フロリダにあるケネディ宇宙センターから打ち上げられ、着地も打ち上げ地点と同じであった。ミッション期間は14日間であった。「コロンビア」が地球へ帰還した後、次の2つの問題を調べた。(1)大腸菌ミュータントに対する高線エネルギー付与宇宙放射線の致死と突然変異性(2)高線エネルギー付与宇宙放射線とpZ189DNAの突然変異の関係。大腸菌KMBL3835(野生株)、KY383(lexA-)、KY385(recA-)、KY386(uvrA-)では、宇宙試料の細胞生存能力と地上試料の細胞生存能力には、殆ど差は見られなかった。同様にE.coil変異種の変異頻度の比率は宇宙と地上での差は少なかった。さらにpZ189DNA宇宙試料の生存能力とsupF遺伝子の突然変異頻度は、地上試料の場合と変らなかった。","subitem_description_type":"Abstract"}]},"item_3_description_17":{"attribute_name":"抄録（英）","attribute_value_mlt":[{"subitem_description":"A space experiment conducted during the 2nd International Microgravity Laboratory Mission (IML-2) project was participated in. This study was designed to investigate the effect of high LET (Linear Energy Transfer) cosmic radiations on living organisms by loading the 'Realtime Radiation Monitoring Device (RRMD)' and 'Radiation Dosimeter' with biological samples, and performing post-flight analysis. As one of preparing procedure of biological samples, the preliminary experiment was performed on earth using Escherichia coli irradiated with Ultraviolet (UV) light. After preparing the biological samples, which were dried E. coli DNA (Deoxyribonucleic Acid) repair-deficient mutants and the shuttle vector plasmid pZ189 DNA, they were placed in the Dosimeters and in the RRMD, which was a Biospecimen Box sandwiched between 'Harzlas' plastic radiation detectors. These were then loaded into the space shuttle 'Columbia' while the identical box and dosimeters were let at the NASA John F. Kennedy Space Center (KSC) as a control on earth. 'Columbia (flight No. STS-65)' was launched from KSC in Florida, USA on July 9, 1994, and landing site was the same place as launch. Mission duration was 14 days. After the return of 'Columbia' to earth, the following two subjects were studied; (1) the lethality and mutagenicity of high LET cosmic radiation on E. coli mutants, and (2) the relation between high LET cosmic radiations and the mutation of pZ189 DNA. For E. coli KMBL3835 (wild type), KY383 (lexA(sup -)), KY385 (recA(sup -)) and KY386 (uvrA(sup -)), there was little difference between the cell viabilities of the space samples and those of the ground samples. Likewise, there was little difference between space and ground in the ratios of mutation frequency of the E. coli mutants. Furthermore, the survival and mutation frequency of supF gene of pZ189 DNA space samples did not differ from those of the ground samples.","subitem_description_type":"Other"}]},"item_3_description_32":{"attribute_name":"資料番号","attribute_value_mlt":[{"subitem_description":"資料番号: AA0000271014","subitem_description_type":"Other"}]},"item_3_description_33":{"attribute_name":"レポート番号","attribute_value_mlt":[{"subitem_description":"レポート番号: NASDA-TMR-960004","subitem_description_type":"Other"}]},"item_3_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"宇宙開発事業団"}]},"item_3_publisher_9":{"attribute_name":"出版者（英）","attribute_value_mlt":[{"subitem_publisher":"National Space Development Agency of Japan (NASDA)"}]},"item_3_source_id_21":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1345-7888","subitem_source_identifier_type":"ISSN"}]},"item_3_source_id_24":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AN00364784","subitem_source_identifier_type":"NCID"}]},"item_3_text_6":{"attribute_name":"著者所属","attribute_value_mlt":[{"subitem_text_value":"宇宙開発事業団 宇宙実験グループ"},{"subitem_text_value":"PL学園女子短期大学 植物研究所"},{"subitem_text_value":"PL学園女子短期大学 植物研究所"},{"subitem_text_value":"PL学園女子短期大学 植物研究所"},{"subitem_text_value":"日本原子力研究所 高崎研究所"},{"subitem_text_value":"日本原子力研究所 高崎研究所"},{"subitem_text_value":"奈良県立医科大学"}]},"item_3_text_7":{"attribute_name":"著者所属（英）","attribute_value_mlt":[{"subitem_text_language":"en","subitem_text_value":"National Space Development Agency of Japan Space Experiment Group"},{"subitem_text_language":"en","subitem_text_value":"PL Gakuen Women's Junior College PL Botanical Institute"},{"subitem_text_language":"en","subitem_text_value":"PL Gakuen Women's Junior College PL Botanical Institute"},{"subitem_text_language":"en","subitem_text_value":"PL Gakuen Women's Junior College PL Botanical Institute"},{"subitem_text_language":"en","subitem_text_value":"Japan Atomic Energy Research Institute Takasaki Radiation Chemistry Research Establishment"},{"subitem_text_language":"en","subitem_text_value":"Japan Atomic Energy Research Institute Takasaki Radiation Chemistry Research Establishment"},{"subitem_text_language":"en","subitem_text_value":"Nara Medical University"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"長岡, 俊治"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"原田, 和樹"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"帯屋, 有里乃"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"中野, 立央"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"小林, 泰彦"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"渡辺, 宏"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"大西, 武雄"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Nagaoka, Shunji","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Harada, Kazuki","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Obiya, Yurino","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Nakano, Tatsuya","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kobayashi, Yasuhiko","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Watanabe, Hiroshi","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Onishi, Takeo","creatorNameLang":"en"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-02-10"}],"displaytype":"detail","filename":"00271014.pdf","filesize":[{"value":"682.4 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"00271014.pdf","url":"https://jaxa.repo.nii.ac.jp/record/39723/files/00271014.pdf"},"version_id":"f32923ca-8664-4db4-9a68-b4790672583d"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"LET","subitem_subject_scheme":"Other"},{"subitem_subject":"線エネルギー付与宇宙放射線","subitem_subject_scheme":"Other"},{"subitem_subject":"RRMD","subitem_subject_scheme":"Other"},{"subitem_subject":"リアルタイム放射線モニター装置","subitem_subject_scheme":"Other"},{"subitem_subject":"放射線線量計","subitem_subject_scheme":"Other"},{"subitem_subject":"大腸菌","subitem_subject_scheme":"Other"},{"subitem_subject":"プラスミド","subitem_subject_scheme":"Other"},{"subitem_subject":"IML-2","subitem_subject_scheme":"Other"},{"subitem_subject":"第2次国際微小重力実験室プロジェクト","subitem_subject_scheme":"Other"},{"subitem_subject":"宇宙実験","subitem_subject_scheme":"Other"},{"subitem_subject":"微小重力","subitem_subject_scheme":"Other"},{"subitem_subject":"生物効果","subitem_subject_scheme":"Other"},{"subitem_subject":"DNA","subitem_subject_scheme":"Other"},{"subitem_subject":"大腸菌ミュータント","subitem_subject_scheme":"Other"},{"subitem_subject":"突然変異","subitem_subject_scheme":"Other"},{"subitem_subject":"LET","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"linear energy transfer cosmic radiation","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"RRMD","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"realtime radiation monitoring device","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"radiation dosimeter","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Escherichia coli","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"plasmid","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"IML 2","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"2nd international microgravity laboratory mission project","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"space experiment","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"microgravity","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"effect on living organisms","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"DNA","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Escherichia coli mutant","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"mutation","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"technical report","resourceuri":"http://purl.org/coar/resource_type/c_18gh"}]},"item_title":"宇宙船内における重粒子線による線量計測とその生物効果実験(RADIATION):大腸菌細胞とプラスミドDNAについて","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"宇宙船内における重粒子線による線量計測とその生物効果実験(RADIATION):大腸菌細胞とプラスミドDNAについて"}]},"item_type_id":"3","owner":"1","path":["1893","1934"],"pubdate":{"attribute_name":"公開日","attribute_value":"2015-03-26"},"publish_date":"2015-03-26","publish_status":"0","recid":"39723","relation_version_is_last":true,"title":["宇宙船内における重粒子線による線量計測とその生物効果実験(RADIATION):大腸菌細胞とプラスミドDNAについて"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-06-20T20:29:38.394796+00:00"}