{"created":"2023-06-20T15:11:24.950251+00:00","id":41063,"links":{},"metadata":{"_buckets":{"deposit":"9cee1fee-5515-4873-9062-2d3d538e1323"},"_deposit":{"created_by":1,"id":"41063","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"41063"},"status":"published"},"_oai":{"id":"oai:jaxa.repo.nii.ac.jp:00041063","sets":["1887:1893","1896:1898:1933:1934"]},"author_link":["501244","501245","501247","501246","501243","501249","501242","501248"],"item_3_alternative_title_2":{"attribute_name":"その他のタイトル（英）","attribute_value_mlt":[{"subitem_alternative_title":"Crystal growth of enzymes in microgravity"}]},"item_3_biblio_info_10":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1994-10-20","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"217","bibliographicPageStart":"190","bibliographic_titles":[{"bibliographic_title":"宇宙開発事業団技術報告"},{"bibliographic_title":"NASDA Technical Memorandum","bibliographic_titleLang":"en"}]}]},"item_3_description_16":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"本研究ではスペースシャトル｢エンデバー｣(STS-47)を利用してニワトリ卵白リゾチーム、ウマ骨格筋ミオグロビン、緑膿菌ω-アミノ酸:ピルビン酸アミノ基転移酵素、クモノスカビのリパーゼ、およびヒトのインシュリンの5種類のタンパク質または酵素について微小重力下、20度Cで6種のタンパク質結晶化に関する宇宙実験を実施した。結晶化方法は静置バッチ法を採用し、この原理に基づいて、宇宙実験装置として密閉系のポリカーボネート製酵素結晶化容器を製作した。6種のタンパク質試料のうち、インシュリン以外の5種の試料について結晶が得られ、そのうち4種の試料が単結晶であった。リゾチーム(pH4.5)とω-アミノ酸:ピルビン酸アミノ基転移酵素は結晶学的に良質の大きな単結晶に成長し、筑波の高エネルギー物理学研究所の放射光実験施設(PF)のシンクロトロン放射光を利用してX線結晶学的解析を実施した。","subitem_description_type":"Abstract"}]},"item_3_description_17":{"attribute_name":"抄録（英）","attribute_value_mlt":[{"subitem_description":"The space experiment on the crystallization of six proteins was carried out at 20 C in a microgravity state of space shuttle 'Endeavor' (STS-47). The proteins or enzymes used include fowl albumen lysozyme, horse skeletal muscle myoglobin, Pseudomonas aeruginosa omega-amino acid: pyruvic acid aminotransferase, spiderweb fungal lipase, and human insulin. The stationary batch method was used for crystallization. Based on its principle, a closed system polycarbonate vessel for enzyme crystallization was made as a space experiment device. Five of the six protein samples, except for human insulin, provided crystallized samples. Four of the five samples were single crystals. Lysozyme (pH 4.5) and omega-amino acid: pyruvic acid aminotransferase grew to crystallographically good and large single crystals. They were X-ray crystallographically analyzed using synchrotron radiation of the High Energy Physics Laboratory's Photon Factory (PF) in Tsukuba.","subitem_description_type":"Other"}]},"item_3_description_32":{"attribute_name":"資料番号","attribute_value_mlt":[{"subitem_description":"資料番号: AA0004115006","subitem_description_type":"Other"}]},"item_3_description_33":{"attribute_name":"レポート番号","attribute_value_mlt":[{"subitem_description":"レポート番号: NASDA-TMR-940002 V.1","subitem_description_type":"Other"}]},"item_3_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"宇宙開発事業団"}]},"item_3_publisher_9":{"attribute_name":"出版者（英）","attribute_value_mlt":[{"subitem_publisher":"National Space Development Agency of Japan (NASDA)"}]},"item_3_source_id_21":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1345-7888","subitem_source_identifier_type":"ISSN"}]},"item_3_source_id_24":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AN00364784","subitem_source_identifier_type":"NCID"}]},"item_3_text_6":{"attribute_name":"著者所属","attribute_value_mlt":[{"subitem_text_value":"不二製油"},{"subitem_text_value":"京都大学食糧科学研究所"},{"subitem_text_value":"京都大学食糧科学研究所"},{"subitem_text_value":"University of Alabama at Birmingham"}]},"item_3_text_7":{"attribute_name":"著者所属（英）","attribute_value_mlt":[{"subitem_text_language":"en","subitem_text_value":"Fuji Oil Co Ltd"},{"subitem_text_language":"en","subitem_text_value":"Research Institute of Food Science, Kyoto University"},{"subitem_text_language":"en","subitem_text_value":"Research Institute of Food Science, Kyoto University"},{"subitem_text_language":"en","subitem_text_value":"University of Alabama at Birmingham"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"森田, 雄平"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"相原, 茂夫"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"三上, 文三"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Charles, E. B."}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Morita, Yuhei","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Aihara, Shigeo","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Mikami, Bunzo","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Charles, E. Bugg","creatorNameLang":"en"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-02-10"}],"displaytype":"detail","filename":"04115006.pdf","filesize":[{"value":"1.5 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"04115006.pdf","url":"https://jaxa.repo.nii.ac.jp/record/41063/files/04115006.pdf"},"version_id":"5dadf21b-4323-431b-9bdc-339cb24daa9a"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"微小重力","subitem_subject_scheme":"Other"},{"subitem_subject":"酵素","subitem_subject_scheme":"Other"},{"subitem_subject":"結晶成長","subitem_subject_scheme":"Other"},{"subitem_subject":"スペースシャトル","subitem_subject_scheme":"Other"},{"subitem_subject":"リゾチーム","subitem_subject_scheme":"Other"},{"subitem_subject":"宇宙実験","subitem_subject_scheme":"Other"},{"subitem_subject":"リパーゼ","subitem_subject_scheme":"Other"},{"subitem_subject":"卵白","subitem_subject_scheme":"Other"},{"subitem_subject":"結晶学的解析","subitem_subject_scheme":"Other"},{"subitem_subject":"タンパク","subitem_subject_scheme":"Other"},{"subitem_subject":"構造モデル","subitem_subject_scheme":"Other"},{"subitem_subject":"単結晶","subitem_subject_scheme":"Other"},{"subitem_subject":"緑膿菌","subitem_subject_scheme":"Other"},{"subitem_subject":"インシュリン","subitem_subject_scheme":"Other"},{"subitem_subject":"ミオグロビン","subitem_subject_scheme":"Other"},{"subitem_subject":"microgravity","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"enzyme","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"crystal growth","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"space shuttle","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"lysozyme","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"space experiment","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"lipase","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"albumen","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"crystallographic analysis","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"protein","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"structure model","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"single crystal","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Pseudomonas aeruginosa","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"insulin","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"myoglobin","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"technical report","resourceuri":"http://purl.org/coar/resource_type/c_18gh"}]},"item_title":"微小重力を利用した酵素の結晶成長","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"微小重力を利用した酵素の結晶成長"}]},"item_type_id":"3","owner":"1","path":["1893","1934"],"pubdate":{"attribute_name":"公開日","attribute_value":"2015-03-26"},"publish_date":"2015-03-26","publish_status":"0","recid":"41063","relation_version_is_last":true,"title":["微小重力を利用した酵素の結晶成長"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-06-20T20:27:30.603304+00:00"}