{"created":"2023-06-20T15:11:25.190834+00:00","id":41068,"links":{},"metadata":{"_buckets":{"deposit":"1bfa5ecb-a52d-4d50-a789-3c1d82ead4be"},"_deposit":{"created_by":1,"id":"41068","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"41068"},"status":"published"},"_oai":{"id":"oai:jaxa.repo.nii.ac.jp:00041068","sets":["1887:1893","1896:1898:1933:1934"]},"author_link":["501306","501312","501314","501310","501311","501318","501313","501309","501315","501317","501316","501308","501307","501319"],"item_3_alternative_title_2":{"attribute_name":"その他のタイトル（英）","attribute_value_mlt":[{"subitem_alternative_title":"Separation of the animal cellular by means of free-flow electrophoresis"}]},"item_3_biblio_info_10":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1994-10-20","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"305","bibliographicPageStart":"284","bibliographic_titles":[{"bibliographic_title":"宇宙開発事業団技術報告"},{"bibliographic_title":"NASDA Technical Memorandum","bibliographic_titleLang":"en"}]}]},"item_3_description_16":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"この実験は宇宙環境の特徴を利用して、フリーフロー(無担体)電気泳動法によりバクテリア細胞混合物から単一菌株を効率的に分離することを目的とする。軌道上での実験は予定の日程に従い進行した。飛行期間の延長により実験を1回追加することができた。搭乗科学者と地上支援科学者との連携作業が順調に機能し、搭載実験装置の吸光度検出系の異常を除いて、大きな支障もなく終了した。細胞資料のモデルとしてバクテリアSalmonella typhimuriumの変異株3種を使用した。軌道上との吸光度データが地上に届かなかったため、予備実験は無意味となり、省略して本実験のみ200Vで2回、300Vで1回実施した。各実験の分画試料は、帰還時まで冷蔵保存した。回収試料を地上で解析したところ、200V泳動実験では、明確な吸光度ピークを検出できなかった。これは試料の泳動が不十分のため分離されておらず、また泳動中の試料の希釈によるためと考えられる。300Vでの泳動実験では吸光度の変化が認められた。各分画について生菌数を測定すると、明確な2つのピークが検出できた。これは3種のバクテリアに相当するもので、残る1種は他の1つのピークに重なっていた。宇宙実験の結果は、地上実験とは異なり、3種のバクテリアのうち少なくとも2種は理論通りに泳動し、分離されていたことを示す。これは地上で避けることのできない熱対流が、微小重力の場では消滅したことによると考えられ、実験の目的はある程度達成できたといえる。しかし、実験の成果は十分とはいえず、微小重力場の分離能が地上に勝ると断定するまでには、さらに宇宙実験を重ねることが必要である。","subitem_description_type":"Abstract"}]},"item_3_description_17":{"attribute_name":"抄録（英）","attribute_value_mlt":[{"subitem_description":"Using the characteristics of the outer space environment, this study aims to efficiently isolate single strains from bacteria cell mixture with free-flow (carrier-free) electrophoresis method. The experiments in orbit progressed satisfactory as scheduled. Prolonged flight allowed us to add a experiment. Cooperation between the scientists on board and on the ground worked smoothly. The experiments were completed without serious failure, except for an absorbance detection system defect of the equipment on board. Three variants of Salmonella typhimurium were used as a cell material model. Because absorbance data on orbit were not transmitted, preliminary experiments were discontinued and formal experiments were conducted two times at 200 V and one time at 300 V. Each experiment's fractionated samples were stored in a refrigerator until returning to the Earth. The collected sample analysis on the ground showed no definite absorbance peak in the 200 V electrophoresis experiment. This is probably because of insufficient sample movement and sample dilution during electrophoresis. The 300 V experiment showed the change in absorbance. Measuring live bacterium number revealed two definite peaks, which were related to three kinds of bacteria. Another peak is related to the remaining one bacterium. These space experiment results indicate that, unlike ground experiments, at least two of the three bacteria theoretically moved and were isolated. The lack of thermal convection in micro gravitational field, which is inevitable on the ground, may result in these results. The purpose of this experiment was achieved to some extent. However, the results are not sufficient, and further space experiments are necessary until concluding that isolation power in micro gravitational field is better than that on the ground.","subitem_description_type":"Other"}]},"item_3_description_32":{"attribute_name":"資料番号","attribute_value_mlt":[{"subitem_description":"資料番号: AA0004115011","subitem_description_type":"Other"}]},"item_3_description_33":{"attribute_name":"レポート番号","attribute_value_mlt":[{"subitem_description":"レポート番号: NASDA-TMR-940002 V.1","subitem_description_type":"Other"}]},"item_3_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"宇宙開発事業団"}]},"item_3_publisher_9":{"attribute_name":"出版者（英）","attribute_value_mlt":[{"subitem_publisher":"National Space Development Agency of Japan (NASDA)"}]},"item_3_source_id_21":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1345-7888","subitem_source_identifier_type":"ISSN"}]},"item_3_source_id_24":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AN00364784","subitem_source_identifier_type":"NCID"}]},"item_3_text_6":{"attribute_name":"著者所属","attribute_value_mlt":[{"subitem_text_value":"東京医科歯科大"},{"subitem_text_value":"理化学研究所"},{"subitem_text_value":"三菱重工 神戸造船所"},{"subitem_text_value":"三菱重工 神戸造船所"},{"subitem_text_value":"バイオシステムインターナショナル"},{"subitem_text_value":"北海道大学 農学部"},{"subitem_text_value":"NASDA 宇宙実験グループ"}]},"item_3_text_7":{"attribute_name":"著者所属（英）","attribute_value_mlt":[{"subitem_text_language":"en","subitem_text_value":"Tokyo Medical and Dental University"},{"subitem_text_language":"en","subitem_text_value":"Institute of Physical and Chemical Research"},{"subitem_text_language":"en","subitem_text_value":"Mitsubishi Heavy Industries Ltd Kobe Shipyard and Machinery Works"},{"subitem_text_language":"en","subitem_text_value":"Mitsubishi Heavy Industries Ltd Kobe Shipyard and Machinery Works"},{"subitem_text_language":"en","subitem_text_value":"Biosystem International Co"},{"subitem_text_language":"en","subitem_text_value":"Hokkaido University Faculty of Agriculture"},{"subitem_text_language":"en","subitem_text_value":"National Space Development Agency of Japan Space Experiment Group"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"山口, 登喜夫"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"秋葉, 晄彦"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"高沖, 宗夫"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"西, 敦子"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"松宮, 弘幸"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"富田, 房男"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"長岡, 俊治"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Yamaguchi, Tokio","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Akiba, Akihiko","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Takaoki, Muneo","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Nishi, Atsuko","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Matsumiya, Hiroyuki","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Tomita, Fusao","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Nagaoka, Syunji","creatorNameLang":"en"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-02-10"}],"displaytype":"detail","filename":"04115011.pdf","filesize":[{"value":"651.8 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"04115011.pdf","url":"https://jaxa.repo.nii.ac.jp/record/41068/files/04115011.pdf"},"version_id":"8dd55874-8057-4d30-889a-8ff7e7b0e07f"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"フリーフロー電気泳動法","subitem_subject_scheme":"Other"},{"subitem_subject":"バクテリア","subitem_subject_scheme":"Other"},{"subitem_subject":"細胞分離","subitem_subject_scheme":"Other"},{"subitem_subject":"宇宙環境","subitem_subject_scheme":"Other"},{"subitem_subject":"菌株","subitem_subject_scheme":"Other"},{"subitem_subject":"搭乗科学者","subitem_subject_scheme":"Other"},{"subitem_subject":"変異体","subitem_subject_scheme":"Other"},{"subitem_subject":"吸光度","subitem_subject_scheme":"Other"},{"subitem_subject":"宇宙実験","subitem_subject_scheme":"Other"},{"subitem_subject":"微小重力","subitem_subject_scheme":"Other"},{"subitem_subject":"胞子細胞","subitem_subject_scheme":"Other"},{"subitem_subject":"カビ","subitem_subject_scheme":"Other"},{"subitem_subject":"融合細胞","subitem_subject_scheme":"Other"},{"subitem_subject":"寒天培地","subitem_subject_scheme":"Other"},{"subitem_subject":"易動度","subitem_subject_scheme":"Other"},{"subitem_subject":"free flow electrophoresis","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"bacterium","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"cell dissociation","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"space environment","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"strain","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"payload specialist","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"variant","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"absorbance","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"space experiment","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"microgravity","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"spore cell","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"mold","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"fused cell","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"agar medium","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"mobility","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"technical report","resourceuri":"http://purl.org/coar/resource_type/c_18gh"}]},"item_title":"フリーフロー電気泳動法によるバクテリア細胞の分離","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"フリーフロー電気泳動法によるバクテリア細胞の分離"}]},"item_type_id":"3","owner":"1","path":["1893","1934"],"pubdate":{"attribute_name":"公開日","attribute_value":"2015-03-26"},"publish_date":"2015-03-26","publish_status":"0","recid":"41068","relation_version_is_last":true,"title":["フリーフロー電気泳動法によるバクテリア細胞の分離"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-06-20T20:27:24.472535+00:00"}