{"created":"2023-06-20T15:17:14.912595+00:00","id":47395,"links":{},"metadata":{"_buckets":{"deposit":"a6dbaf07-0f3f-4510-a6b8-e79fd712bcdf"},"_deposit":{"created_by":1,"id":"47395","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"47395"},"status":"published"},"_oai":{"id":"oai:jaxa.repo.nii.ac.jp:00047395","sets":["1398:1408:2123","1887:1893","9:10:2065:2120"]},"author_link":["532759","532751","532768","532760","532776","532761","532778","532767","532770","532779","532784","532769","532774","532752","532773","532766","532771","532755","532772","532781","532757","532750","532762","532782","532765","532775","532777","532754","532753","532756","532780","532764","532763","532783","532758","532749"],"item_3_alternative_title_2":{"attribute_name":"その他のタイトル（英）","attribute_value_mlt":[{"subitem_alternative_title":"A report on the 2017-2019 balloon experiments of the stratospheric bioaerosol sampling (Biopause project)"}]},"item_3_biblio_info_10":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2021-02-26","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"17","bibliographicPageStart":"1","bibliographicVolumeNumber":"JAXA-RR-20-009","bibliographic_titles":[{"bibliographic_title":"宇宙航空研究開発機構研究開発報告: 大気球研究報告"},{"bibliographic_title":"JAXA Research and Development Report","bibliographic_titleLang":"en"}]}]},"item_3_description_16":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Biopauseプロジェクトでは、地球生命圏の上端“biopause”を理解するために成層圏微生物の採取実験を行っている。我々が新規開発した世界初の降下式インパクター型試料採取装置を搭載したJAXA（宇宙航空研究開発機構）の大気球を用いた第一回目となる2016年度実験では、成層圏微粒子の採取に成功した。次のステップとして、成層圏微生物の鉛直分布を把握するため、同時同地点異高度における試料採取を2017年度、並びに2019年度大気球実験にて行った。また、成層圏における培養可能な微生物と非培養な微生物の比率を推定し先行研究との定量的な比較を行うため、培養法分析も同時に行った。実験が成功した2019年度実験の結果から、非培養性微生物を含めた成層圏由来の微生物は一切検出されず、実験時における採取高度では微生物の存在量が検出限界未満であったことが示された。本稿では、2017年度と2019年度にJAXA共同利用実験として実施された本プロジェクトの大気球実験の概要とその分析結果について報告する。","subitem_description_type":"Abstract"}]},"item_3_description_17":{"attribute_name":"抄録（英）","attribute_value_mlt":[{"subitem_description":"The aim of Biopause project is to conduct a sequence of bioaerosol sampling in the stratosphere, in order to determine the location of ''biopause'', the upper boundary of the biosphere. By using JAXA's scientific balloons equipped with our newly developed world's first descending inertial impactor-style sampling device, we managed to collect some stratospheric bioaerosols in 2016. For the next step, to understand a vertical distribution of those stratospheric bioaerosols, we conducted the simultaneous bioaerosol sampling at the same location different altitudes in 2017 and 2019. We also carried out the cultivation analysis to have a quantitative comparison between culturable and unculturable stratospheric microorganisms. The results of a successful experiment in 2019 show the collection of zero number of bioaerosols including unculturable stratospheric microorganisms, which indicates the number of microorganisms at that time and altitudes of sampling was below the detection limit of our experimental methods. In this paper, we report the results from both balloon experiments of the Biopause project in 2017 and 2019.","subitem_description_type":"Other"}]},"item_3_description_18":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"形態: カラー図版あり","subitem_description_type":"Other"}]},"item_3_description_19":{"attribute_name":"内容記述（英）","attribute_value_mlt":[{"subitem_description":"Physical characteristics: Original contains color illustrations","subitem_description_type":"Other"}]},"item_3_description_32":{"attribute_name":"資料番号","attribute_value_mlt":[{"subitem_description":"資料番号: AA2030024001","subitem_description_type":"Other"}]},"item_3_description_33":{"attribute_name":"レポート番号","attribute_value_mlt":[{"subitem_description":"レポート番号: JAXA-RR-20-009","subitem_description_type":"Other"}]},"item_3_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.20637/00047382","subitem_identifier_reg_type":"JaLC"}]},"item_3_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"宇宙航空研究開発機構(JAXA)"}]},"item_3_publisher_9":{"attribute_name":"出版者（英）","attribute_value_mlt":[{"subitem_publisher":"Japan Aerospace Exploration Agency 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experiment","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"extremophile","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"bioaerosol","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"technical 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