{"created":"2023-06-20T14:39:03.254386+00:00","id":5725,"links":{},"metadata":{"_buckets":{"deposit":"6bdc0e0a-6c63-421e-8dbd-14c283976715"},"_deposit":{"created_by":1,"id":"5725","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"5725"},"status":"published"},"_oai":{"id":"oai:jaxa.repo.nii.ac.jp:00005725","sets":["1887:1893","9:789:998:1001"]},"author_link":["28160","28161"],"item_3_alternative_title_2":{"attribute_name":"その他のタイトル（英）","attribute_value_mlt":[{"subitem_alternative_title":"Hydrotropism and auxin-inducible gene expression in roots grown under microgravity conditions"}]},"item_3_biblio_info_10":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2007-03-30","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"11","bibliographicPageStart":"5","bibliographicVolumeNumber":"JAXA-SP-06-033","bibliographic_titles":[{"bibliographic_title":"宇宙航空研究開発機構特別資料: ISS科学プロジェクト室植物生理研究プロジェクトFerulate & Hydro Tropi研究開発報告"},{"bibliographic_title":"JAXA Special Publication: Report of Plant Physiology Research Project in ISS Science Project Office: Ferulate & Hydro Tropi Research and Development","bibliographic_titleLang":"en"}]}]},"item_3_description_16":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"本研究では、微小重力下で根の水分屈性と重力屈性を分離し、水分屈性に対する重力屈性の干渉作用を検証し、それぞれの場合のオーキシン制御遺伝子の発現変化をオーキシン動態として捉え、両屈性におけるオーキシンの役割からそれぞれのメカニズムを明らかにするとともに、微小重力下における根の伸長方向を水分屈性により制御することを可能にするための宇宙実験を実施することを目的としている。そのために本年度は、宇宙実験計画書のベースライン化を行うとともに、これまでの検討・指摘事項を中心に、EMCS(European Modular Cultivation System)用GRAVIチャンバー模型およびCBEF(Cell Biology Experimental Facility)/V-MEU用チャンバーを試作し、供試植物材料としてキュウリ芽生えを用いて水分屈性実験を行った。またクリノスタット実験によって、キュウリ芽生えの根の重力屈性と水分屈性を分離し、両屈性におけるオーキシンの役割を検証した。その結果、本研究目的を達成するためにキュウリ芽生えを用いることが可能であること、キュウリの根の水分屈性にはオーキシンの輸送・作用が重要な役割を果たし、それらを重力屈性から分離して解析するために宇宙実験が有用であることが強く示唆された。一方、シロイヌナズナにおいては、根の水分屈性にオーキシンが重要な役割を果たすが、そのためのオーキシン動態はキュウリの場合とは異なるメカニズムによって制御される可能性が見出された。加えて、根の水分屈性に特異的且つ必須の遺伝子(MIZ1: MIZU-KUSSEI1)を世界ではじめて同定した。","subitem_description_type":"Abstract"}]},"item_3_description_17":{"attribute_name":"抄録（英）","attribute_value_mlt":[{"subitem_description":"Using space environment, we will separate the hydrotropic response mechanism from that of gravitropism for understanding the regulatory mechanisms of root growth orientation and determine whether hydrotropic response can be used for controlling orientation of root growth in microgravity. To achieve these objectives, we established an experimental system with cucumber seedlings and examined the role of auxin in root hydrotropism. In cucumber roots, hydrotropic response was separated from gravitropism by nullifying gravitropic response due to clinorotation. As reported for root gravitropism, it was confirmed that auxin transport and action played important roles in hydrotropism of cucumber roots. Auxin-inducible gene, CsIAA1, was indeed expressed more abundantly on the high-water potential side of the root than the lower-water potential side in the presence of moisture gradient. This implies that there is a competition in auxin redistribution due to gravistimulation and hydrostimulation in cucumber roots, which may accounts for counteracting effects of the two tropisms. Our results suggest that these two counteracting factors can be distinguished in microgravity. On the other hand, Arabidopsis roots may possess a regulatory mechanism for auxin dynamics different from that of gravitropism or cucumber roots. In addition, we identified a gene (MIZ1: MIZU-KUSSEI1) specifically functional and essential for hydrotropism in roots. The MIZ1-involved pathway is therefore independent of gravitropic one. Furthermore, we developed experimental chambers to be used within EMCS (European Modular Cultivation System) or CBEF (Cell Biology Experimental Facility) for hydrotropism experiments of cucumber roots and succeeded in inducing their hydrotropic curvature in the vessels.","subitem_description_type":"Other"}]},"item_3_description_32":{"attribute_name":"資料番号","attribute_value_mlt":[{"subitem_description":"資料番号: AA0063339002","subitem_description_type":"Other"}]},"item_3_description_33":{"attribute_name":"レポート番号","attribute_value_mlt":[{"subitem_description":"レポート番号: JAXA-SP-06-033","subitem_description_type":"Other"}]},"item_3_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"宇宙航空研究開発機構"}]},"item_3_publisher_9":{"attribute_name":"出版者（英）","attribute_value_mlt":[{"subitem_publisher":"Japan Aerospace Exploration Agency (JAXA)"}]},"item_3_source_id_21":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1349-113X","subitem_source_identifier_type":"ISSN"}]},"item_3_source_id_24":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA11984031","subitem_source_identifier_type":"NCID"}]},"item_3_text_6":{"attribute_name":"著者所属","attribute_value_mlt":[{"subitem_text_value":"宇宙航空研究開発機構 宇宙科学研究本部　ISS科学プロジェクト室"}]},"item_3_text_7":{"attribute_name":"著者所属（英）","attribute_value_mlt":[{"subitem_text_language":"en","subitem_text_value":"Japan Aerospace Exploration Agency ISS Science Project Office, Institute of Space and Astronautical Science"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"宇宙航空研究開発機構"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Japan Aerospace Exploration Agency","creatorNameLang":"en"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-01-16"}],"displaytype":"detail","filename":"63339002.pdf","filesize":[{"value":"510.8 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"63339002.pdf","url":"https://jaxa.repo.nii.ac.jp/record/5725/files/63339002.pdf"},"version_id":"34083097-ccfc-444f-a05b-40b70ffc8aa2"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"水分屈性","subitem_subject_scheme":"Other"},{"subitem_subject":"重力屈性","subitem_subject_scheme":"Other"},{"subitem_subject":"微小重力","subitem_subject_scheme":"Other"},{"subitem_subject":"航空宇宙環境","subitem_subject_scheme":"Other"},{"subitem_subject":"オーキシン","subitem_subject_scheme":"Other"},{"subitem_subject":"根","subitem_subject_scheme":"Other"},{"subitem_subject":"遺伝子発現","subitem_subject_scheme":"Other"},{"subitem_subject":"重力効果","subitem_subject_scheme":"Other"},{"subitem_subject":"キュウリ","subitem_subject_scheme":"Other"},{"subitem_subject":"遺伝子","subitem_subject_scheme":"Other"},{"subitem_subject":"hydrotropism","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"gravitropism","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"microgravity","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"aerospace environment","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"auxin","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"root","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"gene expression","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"gravitational effect","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"cucumber","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"gene","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"technical report","resourceuri":"http://purl.org/coar/resource_type/c_18gh"}]},"item_title":"微小重力下における根の水分屈性とオーキシン制御遺伝子の発現","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"微小重力下における根の水分屈性とオーキシン制御遺伝子の発現"}]},"item_type_id":"3","owner":"1","path":["1001","1893"],"pubdate":{"attribute_name":"公開日","attribute_value":"2015-03-26"},"publish_date":"2015-03-26","publish_status":"0","recid":"5725","relation_version_is_last":true,"title":["微小重力下における根の水分屈性とオーキシン制御遺伝子の発現"],"weko_creator_id":"1","weko_shared_id":1},"updated":"2023-06-20T19:40:40.192422+00:00"}