{"created":"2023-06-20T14:39:20.105917+00:00","id":6079,"links":{},"metadata":{"_buckets":{"deposit":"eeb88e13-a7ed-452a-839d-895f89c925be"},"_deposit":{"created_by":1,"id":"6079","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"6079"},"status":"published"},"_oai":{"id":"oai:jaxa.repo.nii.ac.jp:00006079","sets":["1887:1893","9:789:1031:1033"]},"author_link":["30304","30303"],"item_3_alternative_title_2":{"attribute_name":"その他のタイトル（英）","attribute_value_mlt":[{"subitem_alternative_title":"LOH analysis for TK mutants in human cultured cell"}]},"item_3_biblio_info_10":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2006-03-31","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"15","bibliographicPageStart":"5","bibliographicVolumeNumber":"JAXA-SP-05-039","bibliographic_titles":[{"bibliographic_title":"宇宙航空研究開発機構特別資料: 平成17年度ISS科学プロジェクト室放射線生物研究プロジェクト研究開発報告"},{"bibliographic_title":"JAXA Special Publication: FY2005 Research and Development Report on Radiation Biology Research Project at ISS Science Project Group","bibliographic_titleLang":"en"}]}]},"item_3_description_16":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"本研究では、宇宙放射線の遺伝的影響を検出するために、ヒト培養細胞を凍結状態で長期間、国際宇宙ステーションに滞在させるとともに、その期間中に細胞を培養し、再凍結することを計画している。その地上準備研究は大別すると、2つに分けられる。ひとつは、細胞の凍結、解凍、培養、再凍結の一連の過程を、実際に国際宇宙ステーションで行える実験系として確立することである。もうひとつは、実際の宇宙空間で予測される低線量かつ低線量率放射線被ばくによる健康影響の推測に有効な遺伝的影響検出系を確立し、模擬実験系で実証していくことである。このような意図のもとに準備研究を進めたところ、ヒトリンパ芽球細胞TK6を用いたLOH(Loss of Heterozygosity:ヘテロ接合性の喪失)解析システムが、期待通りに有効であることを示唆する実験結果が得られたので、このことを報告する。","subitem_description_type":"Abstract"}]},"item_3_description_17":{"attribute_name":"抄録（英）","attribute_value_mlt":[{"subitem_description":"For estimating the genetic effects of space environmental radiation in this research project, we plan to preserve the human cultured cells as frozen status for a long period in International Space Station (ISS), and further incubate and re-freeze in need during such stay. The preparation experiments on the ground can be classified into two types. One of them is to establish the methodologies how to prepare the cell sample, for example, freezing before the flight mission, incubation and re-freezing in ISS. Another is to develop the sensitive mutation-detection system, which is effective for low-dose and low-dose-rate space environmental radiation. Here, we would like to introduce our current progresses in the above experiments, using human lymphoblastoid TK 6 cell and the loss of heterozygosity (LOH) analysis system.","subitem_description_type":"Other"}]},"item_3_description_32":{"attribute_name":"資料番号","attribute_value_mlt":[{"subitem_description":"資料番号: AA0049800002","subitem_description_type":"Other"}]},"item_3_description_33":{"attribute_name":"レポート番号","attribute_value_mlt":[{"subitem_description":"レポート番号: JAXA-SP-05-039","subitem_description_type":"Other"}]},"item_3_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"宇宙航空研究開発機構"}]},"item_3_publisher_9":{"attribute_name":"出版者（英）","attribute_value_mlt":[{"subitem_publisher":"Japan Aerospace Exploration Agency (JAXA)"}]},"item_3_source_id_21":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1349-113X","subitem_source_identifier_type":"ISSN"}]},"item_3_source_id_24":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA11984031","subitem_source_identifier_type":"NCID"}]},"item_3_text_6":{"attribute_name":"著者所属","attribute_value_mlt":[{"subitem_text_value":"理化学研究所 先端技術開発支援センター"}]},"item_3_text_7":{"attribute_name":"著者所属（英）","attribute_value_mlt":[{"subitem_text_language":"en","subitem_text_value":"Institute of Physical and Chemical Research Advanced Development and Supporting Center"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"谷田貝, 文夫"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Yatagai, Fumio","creatorNameLang":"en"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-01-16"}],"displaytype":"detail","filename":"49800002.pdf","filesize":[{"value":"4.9 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"49800002.pdf","url":"https://jaxa.repo.nii.ac.jp/record/6079/files/49800002.pdf"},"version_id":"c85ac7e3-f94f-4562-8506-62aa1ca54cbf"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"ヒト","subitem_subject_scheme":"Other"},{"subitem_subject":"培養細胞","subitem_subject_scheme":"Other"},{"subitem_subject":"宇宙線","subitem_subject_scheme":"Other"},{"subitem_subject":"航空宇宙環境","subitem_subject_scheme":"Other"},{"subitem_subject":"国際宇宙ステーション","subitem_subject_scheme":"Other"},{"subitem_subject":"変異","subitem_subject_scheme":"Other"},{"subitem_subject":"遺伝子発現","subitem_subject_scheme":"Other"},{"subitem_subject":"ヘテロ接合性","subitem_subject_scheme":"Other"},{"subitem_subject":"凍結","subitem_subject_scheme":"Other"},{"subitem_subject":"human","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"cultured cell","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"cosmic ray","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"aerospace environment","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"International Space Station","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"mutation","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"gene expression","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"heterozygosity","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"freezing","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"technical report","resourceuri":"http://purl.org/coar/resource_type/c_18gh"}]},"item_title":"ヒト培養細胞におけるTK変異体のLOHパターン変化の検出","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"ヒト培養細胞におけるTK変異体のLOHパターン変化の検出"}]},"item_type_id":"3","owner":"1","path":["1033","1893"],"pubdate":{"attribute_name":"公開日","attribute_value":"2015-03-26"},"publish_date":"2015-03-26","publish_status":"0","recid":"6079","relation_version_is_last":true,"title":["ヒト培養細胞におけるTK変異体のLOHパターン変化の検出"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-06-21T08:02:33.249958+00:00"}