{"created":"2023-06-20T14:39:20.460785+00:00","id":6087,"links":{},"metadata":{"_buckets":{"deposit":"5b401e85-3fe8-4c07-9d0d-2c260c8b4e78"},"_deposit":{"created_by":1,"id":"6087","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"6087"},"status":"published"},"_oai":{"id":"oai:jaxa.repo.nii.ac.jp:00006087","sets":["1887:1893","9:789:1031:1035"]},"author_link":["30328","30327"],"item_3_alternative_title_2":{"attribute_name":"その他のタイトル（英）","attribute_value_mlt":[{"subitem_alternative_title":"Gravity response mechanisms in plants searched for proteomics analysis"}]},"item_3_biblio_info_10":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2006-03-31","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"28","bibliographicPageStart":"24","bibliographicVolumeNumber":"JAXA-SP-05-037","bibliographic_titles":[{"bibliographic_title":"宇宙航空研究開発機構特別資料: 平成17年度ISS科学プロジェクト室植物生理研究プロジェクト研究開発報告"},{"bibliographic_title":"JAXA Special Publication: FY2005 Research and Development Report on Plant Physiology Research Project at ISS Science Project Group","bibliographic_titleLang":"en"}]}]},"item_3_description_16":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"近年、多くの生物種のゲノム配列が明らかにされるにつれ、遺伝子発現後の生体内でのタンパク質の動態を明らかにするためのプロテオミクス解析が多く行われている。植物においてもステージ毎や、塩や乾燥などのストレスに対するタンパク質の発現解析がシロイヌナズナ、イネ、トウモロコシ、エンドウを用いて行われている。本研究では、植物の根の重力屈性に関わるタンパク質を解析するために、シロイヌナズナ根およびトウモロコシコルメラ細胞を用いてプロテオミクス解析を行った。その結果、シロイヌナズナ根において、pyruvate dehydrogenase E1 component βsubunit、fructose-bisphosphate aldolaseおよび20S proteasome β subunit E1の3分子種が、電力刺激の経過時間に伴って、一過性的に分子量変化を起こしていることが明らかになった。また、重力刺激を処理すると、シロイヌナズナおよびトウモロコシの両植物とも、リン酸化タンパク質の発現が著しく減少していることも明らかになり、植物の重力屈性機構において、同一タンパク質の分子量変化およびリン酸化タンパク質が、重要な意義を持つ可能性が示唆された。","subitem_description_type":"Abstract"}]},"item_3_description_17":{"attribute_name":"抄録（英）","attribute_value_mlt":[{"subitem_description":"Gravity direction and force exert a profound influence on plant growth. When vertically growing plants are placed in a horizontal orientation, the so-called gravitational stimulation acts on the plants, resulting in the upward and downward growth of the apical shoot and root tip, respectively. This phenomenon in plants is called gravitropism. Proteomics may be the most promising technique to identify the proteins that are induced, repressed, or post-transcriptionally modified during gravity response in plants. In this study, Arabidopsis root apices and Maize columella cells proteins induced and/or changed by gravitational stimulation were analyzed using a proteomic method. As a result, the beta subunit of the E1 component of pyruvate dehydrogenase, fructose bisphosphate aldolase, and 20S proteasome beta subunit E1 protein appeared in two different molecular weight types during gravitational stimulation in Arabidopsis. The transitory changes in molecular weight in these three identified proteins were important findings although their functions in the gravity response remain uncertain. In addition, the phospho-protein was found remarkably decreasing by the gravitational stimulation in Arabidopsis and Maize, so, it was suggested that the phospho-protein was importance in gravity response mechanisms.","subitem_description_type":"Other"}]},"item_3_description_32":{"attribute_name":"資料番号","attribute_value_mlt":[{"subitem_description":"資料番号: AA0049799005","subitem_description_type":"Other"}]},"item_3_description_33":{"attribute_name":"レポート番号","attribute_value_mlt":[{"subitem_description":"レポート番号: JAXA-SP-05-037","subitem_description_type":"Other"}]},"item_3_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"宇宙航空研究開発機構"}]},"item_3_publisher_9":{"attribute_name":"出版者（英）","attribute_value_mlt":[{"subitem_publisher":"Japan Aerospace Exploration Agency (JAXA)"}]},"item_3_source_id_21":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1349-113X","subitem_source_identifier_type":"ISSN"}]},"item_3_source_id_24":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA11984031","subitem_source_identifier_type":"NCID"}]},"item_3_text_6":{"attribute_name":"著者所属","attribute_value_mlt":[{"subitem_text_value":"宇宙航空研究開発機構 宇宙科学研究本部　ISS科学プロジェクト室"}]},"item_3_text_7":{"attribute_name":"著者所属（英）","attribute_value_mlt":[{"subitem_text_language":"en","subitem_text_value":"Japan Aerospace Exploration Agency ISS Science Project Office, Institute of Space and Astronautical Science"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"鎌田, 源司"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kamada, Motoshi","creatorNameLang":"en"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-01-16"}],"displaytype":"detail","filename":"49799005.pdf","filesize":[{"value":"2.1 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"49799005.pdf","url":"https://jaxa.repo.nii.ac.jp/record/6087/files/49799005.pdf"},"version_id":"0e0d2d6c-82f6-41f3-824a-ef57eead2820"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"プロテオミクス","subitem_subject_scheme":"Other"},{"subitem_subject":"重力応答","subitem_subject_scheme":"Other"},{"subitem_subject":"植物","subitem_subject_scheme":"Other"},{"subitem_subject":"植物成長","subitem_subject_scheme":"Other"},{"subitem_subject":"根","subitem_subject_scheme":"Other"},{"subitem_subject":"分子量","subitem_subject_scheme":"Other"},{"subitem_subject":"ペプチド","subitem_subject_scheme":"Other"},{"subitem_subject":"電気泳動","subitem_subject_scheme":"Other"},{"subitem_subject":"proteomics","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"gravitational response","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"plant","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"plant growth","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"root","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"molecular weight","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"peptide","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"electrophoresis","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"technical report","resourceuri":"http://purl.org/coar/resource_type/c_18gh"}]},"item_title":"プロテオミクス解析から探る植物の重力応答機構","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"プロテオミクス解析から探る植物の重力応答機構"}]},"item_type_id":"3","owner":"1","path":["1035","1893"],"pubdate":{"attribute_name":"公開日","attribute_value":"2015-03-26"},"publish_date":"2015-03-26","publish_status":"0","recid":"6087","relation_version_is_last":true,"title":["プロテオミクス解析から探る植物の重力応答機構"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-06-21T08:02:25.778098+00:00"}