Pennsylvania State University Dept. Biochemistry & Molecular Biology
Pennsylvania State University Dept. Biochemistry & Molecular Biology
Pennsylvania State University Dept. Biochemistry & Molecular Biology
Pennsylvania State University Dept. Biochemistry & Molecular Biology
Emerald Diagnostics
宇宙開発事業団
著者所属（英）
Pennsylvania State University Department of Biochemistry and Molecular Biology
Pennsylvania State University Department of Biochemistry and Molecular Biology
Pennsylvania State University Department of Biochemistry and Molecular Biology
Pennsylvania State University Department of Biochemistry and Molecular Biology
Emerald Diagnostics
National Space Development Agency of Japan
出版者
宇宙開発事業団
出版者（英）
National Space Development Agency of Japan (NASDA)
This report describes the results of a Continuous Flow Electrophoresis (CFE) experiment done on STS-65 in which the idea that intracellular Growth Hormone (GH) particles contained in a cell lysate prepared from cultured rat anterior pituitary cells in microgravity might have different electrophoretic mobilities from those in a synchronous ground control cell lysate was tested. Collectively, the results suggested that CFE processing in microgravity was better than on earth; more sample could be processed/time (6x) and more variant forms of GH molecules could be resolved as well. The doing of a pituitary cell CFE experiment had been also hoped, but failure of the hardware required that the actual cell electrophoresis trials be done on earth shortly after Shuttle landing. Data from these experiments showed that spaceflown cells were more electrophoretically mobile than ground control cells, thereby offering evidence for the idea that exposure of cultured cells to microgravity can change their net surface charge, especially when the cells are fed. Collectively, the results from this pituitary cell experiment document the advantage of using coupled cell culture and CFE techniques in the microgravity environment.
Electrophoretic separation of cells and particles from rat pituitary (Pituitary)
00271010.pdf
(1.64MB)
