| Item type |
紀要論文 / Departmental Bulletin Paper(1) |
| 公開日 |
2015-03-26 |
| タイトル |
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タイトル |
リボ核酸の分割分別に関する研究 |
| 言語 |
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言語 |
jpn |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
departmental bulletin paper |
| その他のタイトル（英） |
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その他のタイトル |
Studies on the Fractionation of Ribonucleic Acids |
| 著者 |
三浦, 謹一郎
渡辺, 格
MIURA, Kin-ichiro
WATANABE, Itaru
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| 著者所属 |
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東京大学理工学研究所 |
| 著者所属 |
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東京大学理工学研究所 |
| 著者所属（英） |
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en |
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THE INSTITUTE OF SCIENCE AND TECHNOLOGY UNIVERSITY OF TOKYO |
| 著者所属（英） |
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en |
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THE INSTITUTE OF SCIENCE AND TECHNOLOGY UNIVERSITY OF TOKYO |
| 出版者 |
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出版者 |
東京大学理工学研究所 |
| 書誌情報 |
東京大學理工學研究所報告
巻 12,
号 11,
p. 147-174,
発行日 1958-03-27
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| 抄録（英） |
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内容記述タイプ |
Other |
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内容記述 |
In studying the heterogeneity of ribonueleic acid (RNA), we have fractionated RNA preparations by several mild methods. Yeast RNA prepared by the method of Clarke and Sehryver, whose molecular weight is about 20,000, was fractionated by the use of an ion-exchange resin, Dowex-2 chloride form. Elution was carried out by the stepwise increase in the concentration of sodium chloride up to 5M. Differences in molecular weight, which was determined by the surface film method, but not in nucleotide composition were found among the fractions thus obtained. It was suggested that the molecular size of a fraction determines the ease with which it was eluted by different concentrations of salt. By this method and with this preparation of RNA, we have been unable to obtain the RNA fractions with different nueleotide compositions. Then, cellulose treated with epiehlorohydrin and triethanolamine (ECTEOLA) was tested as an ion-exchanger for fractionating RNA. The results obtained were essentially same as in the case of Dowex-2, but the adsorption of RNA on ECTEOLA adsorbent and the recovery of the eluted RNA from it were a little better than in the case of the resin. Recently, it has been found in our laboratory that highly polymerized RNA can be precipitated from neutral aqueous solution by high concentration of sodiun chloride or by relatively low concentration of magnesium chloride. Highly polymerized yeast RNA prepared by the method of Crest field, Smith, and Allen was fractionated by the precipitation with neutral salts. The fraction soluble in the concentrated salt solution was rich in its guanine and cytosine contents. It seemed that the easily precipitable fraction had higher molecular weight than the soluble one. Thus it is now possible to fractionate any size of RNA into several fractions by the combination of the two methods, i. e. the fractional elution from ionexchanger and the fractional precipitation with neutral salts. |
| ISSN |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
0371-6090 |
| 書誌レコードID |
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収録物識別子タイプ |
NCID |
|
収録物識別子 |
AN00162611 |
| 資料番号 |
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内容記述タイプ |
Other |
|
内容記述 |
資料番号: SA1511823000 |
SA1511823.pdf (2.8 MB)
