Institute of Physical and Chemical Research (RIKEN)
Institute of Physical and Chemical Research (RIKEN)
Nara Medical University
Nara Medical University
Japan Aerospace Exploration Agency (JAXA)
Japan Aerospace Exploration Agency (JAXA)
Japan Space Forum
Japan Space Forum
出版者
宇宙航空研究開発機構宇宙科学研究本部
出版者（英）
Institute of Space and Astronautical Science, Japan Aerospace Exploration Agency (JAXA)
雑誌名
宇宙利用シンポジウム
雑誌名(英)
Space Utilization Research: Proceedings of Space Utilization Symposium
The Twenty-fifth Space Utilization Symposium (January 14-15, 2009: ISAS/JAXA Sagamihara, Japan)
抄録（英）
This paper is aimed to introduce how our developing methodologies for both loss of heterozygosity (LOH) analysis and DNA double-strand break (DSB) repair estimation are applied to detect the biological effects of space radiation. Our space experiments using human cultured cells in Kibou Facility of International Space Station (ISS) are planned to detect mutagenic effects (i), combined effects of radiation with micro gravity (ii), and radioadaptive response (iii). After cell recovery to the earth, we plan to do the following approaches for the above three biological effects: i) an LOH analysis for the mutants induced after the incubation of recovered frozen-cells exposed to low-dose space radiation during the 3 months preservation in ISS, ii) an examination for the existence of combined effects by comparing the mutation inductions between the cells incubated in ISS for ca. １ week under 1 G and those incubated under micro G, and iii) an estimation of the radioadaptive response from determination of the repair efficiencies of DSB introduced at the specific site of chromosome in the recovered cell.
ISSきぼう棟内での細胞培養実験による宇宙放射線影響研究
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