| Item type |
紀要論文 / Departmental Bulletin Paper(1) |
| 公開日 |
2015-03-26 |
| タイトル |
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|
タイトル |
超遠心機によるタバコ・モザイク・バイラスの精製の研究 |
| 言語 |
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|
言語 |
jpn |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
departmental bulletin paper |
| その他のタイトル（英） |
|
|
その他のタイトル |
Study on the Ultracentrifugal Purification of Tobacco Mosaic Virus |
| 著者 |
渡辺, 格
川出, 由己
中村, 正好
WATANABE, Itaru
KAWADE, Yoshimi
NAKAMURA, Masayoshi
|
| 著者所属 |
|
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東京大学理工学研究所 |
| 著者所属 |
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東京大学理工学研究所 |
| 著者所属 |
|
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|
東京大学理工学研究所 |
| 著者所属（英） |
|
|
|
en |
|
|
THE INSTITUTE OF SCIENCE AND TECHNOLOGY, UNIVERSITY OF TOKYO |
| 著者所属（英） |
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|
en |
|
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THE INSTITUTE OF SCIENCE AND TECHNOLOGY, UNIVERSITY OF TOKYO |
| 著者所属（英） |
|
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en |
|
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THE INSTITUTE OF SCIENCE AND TECHNOLOGY, UNIVERSITY OF TOKYO |
| 出版者 |
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出版者 |
東京大学理工学研究所 |
| 書誌情報 |
東京大學理工學研究所報告
巻 8,
号 3,
p. 99-113,
発行日 1954-07-30
|
| 抄録（英） |
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内容記述タイプ |
Other |
|
内容記述 |
Procedures for the purification of tobacco moasic virus (TMV) from tobacco leaves by several cycles of alternate high and low speed centrifugations using an air-driven ultracentrifuge first constructed in Japan recently are described in detail. Colored impurities were easily removed when White Burley tobacco plants were used as the starting material. The solvents used for dissolving pellets of TMV were mainly 0.1M phosphate buffer at pH7.0, followed by 0.01M buffer. The properties of the purified products were examined by various means including biological assay (preliminary) , chemical analyses, electrophoresis, diffusion, sedimentation velocity, viscosity and electron micrography. It was shown that chemical purity was satisfactory, but the homogeneity with respect to the length of the TMV particle was not always good, as judged by electron micrographs and sedimentation patterns. Especially, rod particles shorter than "monomer" (300mμ in length) were always observed in electron micrographs, sometimes existing in large proportions, and the possible causes of their occurrence were discussed. It is presumed that some of them are produced during preparation of the specimen for the electron microscopic observation. Some have probably existed in the original juice. Although a preparation showed a very sharp peak in sedimentation pattern, another preparation, which was purified carefully, revealed two components in sedimentation measurements performed immediately after the purification. Thus, further investigations seem necessary to establish a reliable method to obtain highly homogeneous preparations. |
| ISSN |
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|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
0371-6090 |
| 書誌レコードID |
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|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AN00162611 |
| 資料番号 |
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|
内容記述タイプ |
Other |
|
内容記述 |
資料番号: SA2410210000 |
SA2410210.pdf (2.0 MB)
